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Literature summary for 3.1.1.5 extracted from

  • Ichimasa, M.; Morooka, T.; Niimura, T.
    Purification and properties of a membrane-bound phospholipase B from baker's yeast (Saccharomyces cerevisiae) (1984), J. Biochem., 95, 137-145.
    View publication on PubMed
Search for more literature for 3.1.1.5

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate 0.1%, strongly stimulates phospholipase B activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
 Saccharomyces cerevisiae
deoxycholate
-
 Saccharomyces cerevisiae
Fe3+
-
 Saccharomyces cerevisiae
Hg2+ 5 mM, inhibits phospholipase B activity by 67%, no inhibition of lysophospholipase activity Saccharomyces cerevisiae
SDS
-
 Saccharomyces cerevisiae
Triton X-100
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 phosphatidylcholine
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane tightly bound to membrane Saccharomyces cerevisiae 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
330000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification
-
 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
 phospholipase B activity Saccharomyces cerevisiae
193
-
 lysophospholipase activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-acyl-sn-glycero-3-phosphocholine + H2O
-
 Saccharomyces cerevisiae fatty acid + glycero-3-phosphocholine
-
 ?
2-acylglycerophosphocholine + H2O 2-acyl-sn-glycero-3-phosphocholine Saccharomyces cerevisiae fatty acid + glycerylphosphocholine
-
 ?
phosphatidylcholine + H2O phospholipase B activity, hydrolyzes the acyl ester bonds sequentially, first the 2-acyl group and then the 1-acyl group Saccharomyces cerevisiae lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4
-
 Saccharomyces cerevisiae