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Literature summary for 3.1.1.5 extracted from
- Purification and some properties of soluble phospholipase B from baker's yeast (Saccharomyces cerevisiae) (1985), Agric. Biol. Chem., 49, 1083-1089.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | 0.1%, strongly stimulates phospholipase B activity | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholate | 0.1% sodium deoxycholate, complete inhibition of lysophospholipase activity | Saccharomyces cerevisiae | |
| Fe3+ | - |
Saccharomyces cerevisiae | |
| SDS | - |
Saccharomyces cerevisiae | |
| Triton X-100 | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
lysophosphatidylcholine | - |
Saccharomyces cerevisiae | |
| 0.63 | - |
phosphatidylcholine | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 280000 | 420000 | gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-acyl-sn-glycero-3-phosphocholine + H2O | - |
Saccharomyces cerevisiae | fatty acid + glycero-3-phosphocholine | - |
? | |
| 2-acylglycerophosphocholine + H2O | 2-acyl-sn-glycero-3-phosphocholine | Saccharomyces cerevisiae | fatty acid + glycerylphosphocholine | - |
? | |
| lysophosphatidylcholine + H2O | - |
Saccharomyces cerevisiae | glycerophosphorylcholine + unesterified fatty acid | - |
? | |
| phosphatidylcholine + H2O | phospholipase B activity, hydrolyzes the acyl ester bonds sequentially, first the 2-acyl group and then the 1-acyl group | Saccharomyces cerevisiae | lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate | - |
? | |
| phosphatidylethanolamine + H2O | - |
Saccharomyces cerevisiae | lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate | - |
? | |
| phosphatidylinositol + H2O | - |
Saccharomyces cerevisiae | lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate | - |
? | |
| phosphatidylserine + H2O | - |
Saccharomyces cerevisiae | lysophosphatidylserine + glycerophosphorylserine + a carboxylate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4 | - |
Saccharomyces cerevisiae |
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