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Literature summary for 3.1.1.5 extracted from

  • Oishi, H.; Tsuda, S.; Watanabe, Y.; Tamai, Y.
    Purification and some properties of phospholipase B from Schizosaccharomyces pombe (1996), Biosci. Biotechnol. Biochem., 60, 1087-1092.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate 0.1%, stimulates Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
cetyltrimethylammonium bromide
-
Schizosaccharomyces pombe
EDTA
-
Schizosaccharomyces pombe
Fe2+
-
Schizosaccharomyces pombe
Fe3+
-
Schizosaccharomyces pombe
SDS
-
Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ CaCl2, 10 mM, stimulates Schizosaccharomyces pombe
CoCl2 10 mM, stimulates Schizosaccharomyces pombe
CuSO4 10 mM, stimulates Schizosaccharomyces pombe
HgCl2 10 mM, stimulates Schizosaccharomyces pombe
MgCl2 10 mM, stimulates Schizosaccharomyces pombe
MnCl2 10 mM, stimulates Schizosaccharomyces pombe
NiSO4 10 mM, stimulates Schizosaccharomyces pombe
ZnCl2 10 mM, stimulates Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
gel filtration Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Schizosaccharomyces pombe

Purification (Commentary)

Purification (Comment) Organism
-
Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.71
-
-
Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O 249% of the activity with phosphatidylcholine Schizosaccharomyces pombe glycerophosphorylcholine + unesterified fatty acid
-
?
lysophosphatidylethanolamine + H2O 27% of the activity with phosphatidylcholine Schizosaccharomyces pombe fatty acid + glycerylphosphoethanolamine
-
?
phosphatidic acid + H2O 62% of the activity with phosphatidylcholine Schizosaccharomyces pombe ?
-
?
phosphatidylcholine + H2O
-
Schizosaccharomyces pombe lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
?
phosphatidylinositol + H2O 819% of the activity with phosphatidylcholine Schizosaccharomyces pombe lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
-
?
phosphatidylserine + H2O 22% of the activity with phosphatidylcholine Schizosaccharomyces pombe lysophosphatidylserine + glycerophosphorylserine + a carboxylate
-
?

Subunits

Subunits Comment Organism
dimer 2 * or 3 x * 100000-150000, SDS-PAGE Schizosaccharomyces pombe
trimer 2 * or 3 x * 100000-150000, SDS-PAGE Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Schizosaccharomyces pombe

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30 min, 95% loss of activity Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
Schizosaccharomyces pombe

pH Range

pH Minimum pH Maximum Comment Organism
2.2 3 pH 2.2: about 70% of maximal activity, pH 3.0: about 65% of maximal activity Schizosaccharomyces pombe

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2 6.5 stable between pH 2.2 and pH 6.5 Schizosaccharomyces pombe