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Literature summary for 3.1.1.5 extracted from

  • Sharma, A.; Meena, K.; Kanwar, S.
    Molecular characterization and bioinformatics studies of a lipase from Bacillus thermoamylovorans BHK67 (2018), Int. J. Biol. Macromol., 107, 2131-2140 .
    View publication on PubMed
Search for more literature for 3.1.1.5

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile
-
 Caldibacillus thermoamylovorans
DMSO
-
 Caldibacillus thermoamylovorans
ethanol
-
 Caldibacillus thermoamylovorans
iso-propanol
-
 Caldibacillus thermoamylovorans
methanol
-
 Caldibacillus thermoamylovorans
n-butanol
-
 Caldibacillus thermoamylovorans
n-heptane
-
 Caldibacillus thermoamylovorans
n-hexane
-
 Caldibacillus thermoamylovorans
n-octane
-
 Caldibacillus thermoamylovorans
n-Propanol
-
 Caldibacillus thermoamylovorans
xylene
-
 Caldibacillus thermoamylovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.7
-
 4-nitrophenyl palmitate at pH 7.5 and 55°C Caldibacillus thermoamylovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Caldibacillus thermoamylovorans

Organism

Organism UniProt Comment Textmining
Caldibacillus thermoamylovorans
-
-
-
Caldibacillus thermoamylovorans BHK67
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE anion exchange column chromatography Caldibacillus thermoamylovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Caldibacillus thermoamylovorans BHK67 4-nitrophenol + acetate
-
 ?
4-nitrophenyl benzoate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + benzoate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Caldibacillus thermoamylovorans BHK67 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl formate + H2O worst substrate Caldibacillus thermoamylovorans 4-nitrophenol + formate
-
 ?
4-nitrophenyl myristate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + myristate
-
 ?
4-nitrophenyl myristate + H2O
-
 Caldibacillus thermoamylovorans BHK67 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O best substrate Caldibacillus thermoamylovorans 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O best substrate Caldibacillus thermoamylovorans BHK67 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl stearate + H2O second best substrate Caldibacillus thermoamylovorans 4-nitrophenol + stearate
-
 ?
4-nitrophenyl stearate + H2O second best substrate Caldibacillus thermoamylovorans BHK67 4-nitrophenol + stearate
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 25000, SDS-PAGE Caldibacillus thermoamylovorans

Synonyms

Synonyms Comment Organism
LPL
-
 Caldibacillus thermoamylovorans
LYPLA1
-
 Caldibacillus thermoamylovorans
lysophospholipase I
-
 Caldibacillus thermoamylovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme has a half-life of 10.5 h, 3.7 h and 0.5 h at 55°C, 65°C and 75°C, respectively Caldibacillus thermoamylovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
227.3
-
 4-nitrophenyl palmitate at pH 7.5 and 55°C Caldibacillus thermoamylovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Caldibacillus thermoamylovorans