Cloned (Comment) | Organism |
---|---|
expression of N- and C-FLAG-tagged wild-type human PAF-AH, expression of FLAG-tagged human wild-type PAF-AH and of the engineered PAF-AH mutant in Escherichia coli. A region located at the N-terminal end of PAF-AH regulates expression levels | Mus musculus |
expression of N- and C-FLAG-tagged wild-type mouse PAF-AH, expression of FLAG-tagged human wild-type PAF-AH and of the engineered PAF-AH mutant in Escherichia coli. A region located at the N-terminal end of PAF-AH regulates expression levels | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | replacement of human PAF-AH residues 42-100 with mouse PAF-AH amino acids 41-99, resulting in construct m41-99H, dramatically increases protein and enzymatic activity expression. Conversely, when mouse cDNA N-terminal residues are replaced with the equivalent human sequences, resulting in construct h42-100M, the levels of expression are significantly decreased | Mus musculus |
additional information | replacement of human PAF-AH residues 42-100 with mouse PAF-AH amino acids 41-99, resulting in construct m41-99H, dramatically increases protein and enzymatic activity expression. Conversely, when mouse cDNA N-terminal residues are replaced with the equivalent human sequences, resulting in construct h42-100M, the levels of expression are significantly decreased | Homo sapiens |
N59Q | site-directed mutagenesis, the mutant and wild-type mouse PAFAH expression levels are similar | Mus musculus |
N75Q | site-directed mutagenesis, the mutant and wild-type mouse PAFAH expression levels are similar | Mus musculus |
S61P/P63S | site-directed mutagenesis, the mutant and wild-type mouse PAFAH expression levels are similar | Mus musculus |
General Stability | Organism |
---|---|
human PAF-AH protein is rapidly degraded after cycloheximide treatment of cells, a large portion of immunoreactive precursors are inactive and short-lived | Homo sapiens |
mouse PAF-AH species displays remarkable stability, and enzymatic activity continues to increase after cycloheximide addition due to processing of inactive precursors to fully active protein | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted enzyme | Homo sapiens | - |
- |
extracellular | secereted enzyme | Mus musculus | - |
- |
intracellular | - |
Mus musculus | 5622 | - |
intracellular | - |
Homo sapiens | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Mus musculus | - |
plasma | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
PAF-acetylhydrolase | - |
Mus musculus |
PAF-acetylhydrolase | - |
Homo sapiens |
PAF-AH | - |
Mus musculus |
PAF-AH | - |
Homo sapiens |
plasma platelet-activating factor acetylhydrolase | - |
Mus musculus |
plasma platelet-activating factor acetylhydrolase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | higher expression of mouse compared with human PAF-AH is not due to variations in catalytic efficiency. A region located at the N-terminal end of PAF-AH regulates expression levels, the degree of hydrophobicity and polarity of the N-terminal region correlates with the level of PAF-AH expression | Mus musculus |
additional information | higher expression of mouse compared with human PAF-AH is not due to variations in catalytic efficiency. A region located at the N-terminal end of PAF-AH regulates expression levels, the degree of hydrophobicity and polarity of the N-terminal region correlates with the level of PAF-AH expression | Homo sapiens |