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Literature summary for 3.1.1.45 extracted from

  • Cheah, E.; Ashley, G.W.; Gary, J.; Ollis, D.
    Catalysis by dienelactone hydrolase: a variation on the protease mechanism (1993), Proteins Struct. Funct. Genet., 16, 64-78.
    View publication on PubMed
Search for more literature for 3.1.1.45

Crystallization (Commentary)

Crystallization (Comment) Organism
inhibitor, dienelactam, bound to DLH C123S Pseudomonas knackmussii

Protein Variants

Protein Variants Comment Organism
C123S
-
 Pseudomonas knackmussii

Inhibitors

Inhibitors Comment Organism Structure
dienelactam competitive inhibitor; inhibitor binding studies Pseudomonas knackmussii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas knackmussii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
C123S DLH Pseudomonas knackmussii

Reaction

Reaction Comment Organism Reaction ID
4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate mechanism Pseudomonas knackmussii
a
4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate similar catalytic triads are found in alpha/beta hydrolase fold enzymes: DLH is the simplest one Pseudomonas knackmussii
a
4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases Pseudomonas knackmussii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ?
-
 ?
cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
additional information DLH unable to hydrolyze lactams which closely resemble its substrate, dienelactam is not a substrate, DLH has lost the ability to hydrolyze amides Pseudomonas knackmussii ?
-
 ?
trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

Subunits Comment Organism
monomer alpha,beta protein Pseudomonas knackmussii

Synonyms

Synonyms Comment Organism
DLH
-
 Pseudomonas knackmussii