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Literature summary for 3.1.1.45 extracted from

  • Bruckmann, M.; Blasco, R.; Timmis, K.N.; Pieper, D.H.
    Detoxification of protoanemonin by dienelactone hydrolase (1998), J. Bacteriol., 180, 400-402.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.45

Inhibitors

Inhibitors Comment Organism Structure
protoanemonin competitive inhibitor of the transformation of cis-dienelactone Pseudomonas knackmussii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.381
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii
0.415
-
 protoanemonin
-
 Pseudomonas knackmussii
1.8
-
 trans-4-Carboxymethylenebut-2-en-4-olide
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Burkholderia cepacia
-
 ?
-
 ?
additional information Pseudomonas knackmussii it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
 Pseudomonas cepacia
-
Pseudomonas knackmussii
-
-
-
Pseudomonas putida
-
 RW10, trans-dienelactone hydrolase
-

Purification (Commentary)

Purification (Comment) Organism
3-chlorobenzoate-grown cells Pseudomonas knackmussii
partial Burkholderia cepacia
RW10 partial Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases Pseudomonas knackmussii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.58
-
 substrate protoanemonin Pseudomonas knackmussii
58.8
-
 substrate cis-4-carboxymethylenebut-2-en-4-olide Pseudomonas knackmussii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Burkholderia cepacia 4-oxohex-2-enedioate
-
 ?
cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
cis-4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas putida RW10 not a substrate to a significant extent Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
additional information
-
 Burkholderia cepacia ?
-
 ?
additional information it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation Pseudomonas knackmussii ?
-
 ?
protoanemonin + H2O
-
 Pseudomonas knackmussii cis-acetylacrylate
-
 ?
protoanemonin + H2O activity of cis-dienelactone hydrolase with 50 microM protoanemonin is 0.08% of that observed with 50 microM cis-dienelactone, cis-4-carboxymethylenebut-2-en-4-olide Burkholderia cepacia cis-acetylacrylate
-
 ?
trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Pseudomonas putida
monomer
-
 Burkholderia cepacia
monomer 1 * 28000-29000, SDS-PAGE Pseudomonas knackmussii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.08
-
 protoanemonin
-
 Pseudomonas knackmussii
260
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii