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Literature summary for 3.1.1.45 extracted from
- Novel metal-binding site of Pseudomonas reinekei MT1 trans-dienelactone hydrolase (2009), Biochem. Biophys. Res. Commun., 390, 1345-1348.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a strep-tagged trans-DLH fusion protein in Escherichia coli | Pseudomonas reinekei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D107A | mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity | Pseudomonas reinekei |
| D107A/H111A | mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity | Pseudomonas reinekei |
| E294A | mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity | Pseudomonas reinekei |
| H111A | mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity | Pseudomonas reinekei |
| H281A | mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity | Pseudomonas reinekei |
| Q105A | mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity | Pseudomonas reinekei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | increases activity by the exchange of Zn2+ by Mn2+ | Pseudomonas reinekei |  |
| Zn2+ | Zn-dpendent hydrolase, activity can be modulated by the exchange of Zn2+ by Mn2+ | Pseudomonas reinekei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas reinekei | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-dienelactone + H2O | - |
Pseudomonas reinekei | maleylacetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| trans-dienelactone hydrolase | - |
Pseudomonas reinekei |
| trans-DLH | - |
Pseudomonas reinekei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas reinekei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Pseudomonas reinekei |
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Release 2023.1 (January 2023)
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