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Literature summary for 3.1.1.45 extracted from

  • Blasco, R.; Ramos, J.L.; Wittich, R.M.
    Pseudomonas aeruginosa strain RW41 mineralizes 4-chlorobenzenesulfonate, the major polar by-product from DDT manufacturing (2008), Environ. Microbiol., 10, 1591-1600.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme induction by 4-chlorobenzenesulfonic acid is required for enzyme expression and activity Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview ?
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?
trans-dienelactone + H2O Pseudomonas aeruginosa specific for the trans-isomer, no activity with the cis-isomer maleylacetate
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?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
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Pseudomonas aeruginosa RW41
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Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by anion exchange chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
substrate trans-dienelactone, crude enzyme extract of cells induced by 4-chlorobenzenesulfonic acid Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview Pseudomonas aeruginosa ?
-
?
additional information no activity with protoanemonin Pseudomonas aeruginosa ?
-
?
trans-dienelactone + H2O specific for the trans-isomer, no activity with the cis-isomer Pseudomonas aeruginosa maleylacetate
-
?

Synonyms

Synonyms Comment Organism
dienelactone hydrolase
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Pseudomonas aeruginosa
rans-DLH
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Pseudomonas aeruginosa