Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C123S | 100% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
C123S/R206A | 65% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36A/C123S | no activity activity towards alpha-naphthyl acetate | Escherichia coli |
E36D | 104% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S | 183% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/A134S/S208G/A229V/K234R | 256% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/A134T/A229V | 164% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/A205D | 217% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/A205D/A229V | 222% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/A205D/A229V | 224% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/A205D/A229V | 230% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/A229V | 187% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/F173A | no activity activity towards alpha-naphthyl acetate | Escherichia coli |
E36D/C123S/G211D/A229V | 172% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/G211D/K234N | 205% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/C123S/R206T/A229V | 122% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/R105H/C123S/G211D/K234N | 233% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/R45Q/C123S | 172% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/R45Q/C123S/A205D/A229V | 148% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36D/R81A/C123S/R206A | 114% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
E36N/C123S | 140% activity towards alpha-naphthyl acetate compared with the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethylsulfonyl fluoride | PMSF, mimics the transition state of the reaction | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
p-nitrophenyl acetate | E36D,C123S,A134S,S208G,A229V,K234R mutant enzyme, pH 7.0 | Escherichia coli | |
0.008 | - |
p-nitrophenyl acetate | E36D,C123S mutant enzyme, pH 7.0 | Escherichia coli | |
0.008 | - |
p-nitrophenyl acetate | E36D,R105H,C123S,G211D,K234N mutant enzyme, pH 7.0 | Escherichia coli | |
0.02 | - |
Alpha-naphthyl acetate | E36D,C123S,A134S,S208G,A229V,K234R mutant enzyme, pH 7.0 | Escherichia coli | |
0.021 | - |
p-nitrophenyl acetate | C123S mutant enzyme, pH 7.0 | Escherichia coli | |
0.023 | - |
Alpha-naphthyl acetate | E36D,C123S mutant enzyme, pH 7.0 | Escherichia coli | |
0.032 | - |
Alpha-naphthyl acetate | E36D,R105H,C123S,G211D,K234N mutant enzyme, pH 7.0 | Escherichia coli | |
0.074 | - |
Alpha-naphthyl acetate | C123S mutant enzyme, pH 7.0 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxymethylene-but-2-ene-4-olide + H2O | Escherichia coli | third enzyme of the halocatechol branch of the beta-ketoadipate pathway, catalyses the hydrolysis of both, (E) and (Z) dienelactone to maleyl acetate | (2E)-4-oxohex-2-enedioic acid | - |
? | |
4-carboxymethylene-but-2-ene-4-olide + H2O | Escherichia coli DH5-alpha | third enzyme of the halocatechol branch of the beta-ketoadipate pathway, catalyses the hydrolysis of both, (E) and (Z) dienelactone to maleyl acetate | (2E)-4-oxohex-2-enedioic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain DH5alpha | - |
Escherichia coli DH5-alpha | - |
strain DH5alpha | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxymethylene-but-2-ene-4-olide + H2O | third enzyme of the halocatechol branch of the beta-ketoadipate pathway, catalyses the hydrolysis of both, (E) and (Z) dienelactone to maleyl acetate | Escherichia coli | (2E)-4-oxohex-2-enedioic acid | - |
? | |
4-carboxymethylene-but-2-ene-4-olide + H2O | third enzyme of the halocatechol branch of the beta-ketoadipate pathway, catalyses the hydrolysis of both, (E) and (Z) dienelactone to maleyl acetate | Escherichia coli DH5-alpha | (2E)-4-oxohex-2-enedioic acid | - |
? | |
alpha-naphthyl acetate + H2O | - |
Escherichia coli | 1-naphthol + acetate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Escherichia coli DH5-alpha | 1-naphthol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Escherichia coli | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Escherichia coli DH5-alpha | p-nitrophenol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dienelactone hydrolase | - |
Escherichia coli |
DLH | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
p-nitrophenyl acetate | E36D,C123S mutant enzyme, pH 7.0 | Escherichia coli | |
0.24 | - |
Alpha-naphthyl acetate | E36D,C123S,A134S,S208G,A229V,K234R mutant enzyme, pH 7.0 | Escherichia coli | |
1.6 | - |
Alpha-naphthyl acetate | E36D,C123S mutant enzyme, pH 7.0 | Escherichia coli | |
2.05 | - |
Alpha-naphthyl acetate | E36D,R105H,C123S,G211D,K234N mutant enzyme, pH 7.0 | Escherichia coli | |
2.9 | - |
p-nitrophenyl acetate | C123S mutant enzyme, pH 7.0 | Escherichia coli | |
63.4 | - |
Alpha-naphthyl acetate | C123S mutant enzyme, pH 7.0 | Escherichia coli | |
71.2 | - |
Alpha-naphthyl acetate | E36D,C123S mutant enzyme, pH 7.0 | Escherichia coli | |
86.2 | - |
Alpha-naphthyl acetate | E36D,R105H,C123S,G211D,K234N mutant enzyme, pH 7.0 | Escherichia coli | |
97.9 | - |
p-nitrophenyl acetate | E36D,C123S mutant enzyme, pH 7.0 | Escherichia coli | |
98.1 | - |
Alpha-naphthyl acetate | E36D,C123S,A134S,S208G,A229V,K234R mutant enzyme, pH 7.0 | Escherichia coli | |
107.7 | - |
p-nitrophenyl acetate | C123S mutant enzyme, pH 7.0 | Escherichia coli | |
120 | - |
Alpha-naphthyl acetate | C123S mutant enzyme, pH 7.0 | Escherichia coli | |
123 | - |
p-nitrophenyl acetate | E36D,R105H,C123S,G211D,K234N mutant enzyme, pH 7.0 | Escherichia coli | |
126.8 | - |
p-nitrophenyl acetate | E36D,C123S,A134S,S208G,A229V,K234R mutant enzyme, pH 7.0 | Escherichia coli | |
138 | - |
p-nitrophenyl acetate | E36D,C123S,A134S,S208G,A229V,K234R mutant enzyme, pH 7.0 | Escherichia coli |