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Literature summary for 3.1.1.45 extracted from

  • Walker, I.; Easton, C.J.; Ollis, D.L.
    Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase (2000), Chem. Commun. (Camb.), 2000, 671-672.
No PubMed abstract available
Search for more literature for 3.1.1.45

Protein Variants

Protein Variants Comment Organism
C123S site-directed mutagenesis, mutation of the active site cysteine to a serine, giving a catalytic triad found in serine proteases, completely changes the catalytic activity to a dienelactone isomerase Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 DLH type III
-
Pseudomonas sp. B13
-
 DLH type III
-

Reaction

Reaction Comment Organism Reaction ID
4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate wild-type enzyme catalytic triad: Cys-His-Asn, mutant enzyme catalytic triad: Ser-His-Asn Pseudomonas sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
E/Z-dienelactone + H2O the reaction is reversible when performed by the mutant enzyme C123S Pseudomonas sp. maleylacetate
-
 ?
E/Z-dienelactone + H2O the reaction is reversible when performed by the mutant enzyme C123S Pseudomonas sp. B13 maleylacetate
-
 ?
additional information the mutant C123S catalyzes the isomerase reaction which changes the E-isomer to Z-isomer and vice versa Pseudomonas sp. ?
-
 ?
additional information the mutant C123S catalyzes the isomerase reaction which changes the E-isomer to Z-isomer and vice versa Pseudomonas sp. B13 ?
-
 ?
trans-dienelactone + H2O
-
 Pseudomonas sp. maleylacetate
-
 ?
trans-dienelactone + H2O
-
 Pseudomonas sp. B13 maleylacetate
-
 ?
Z-dienelactone + H2O
-
 Pseudomonas sp. maleyl acetate
-
 ?
Z-dienelactone + H2O
-
 Pseudomonas sp. B13 maleyl acetate
-
 ?

Synonyms

Synonyms Comment Organism
dienelactone hydrolase
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 Z-dienelactone mutant C123S, pH 7.0, 24°C Pseudomonas sp.
0.63
-
 E-dienelactone mutant C123S, pH 7.0, 24°C Pseudomonas sp.
6.08
-
 E-dienelactone mutant C123S, pH 7.0, 24°C Pseudomonas sp.
14
-
 E-dienelactone wild-type enzyme, pH 7.0, 24°C Pseudomonas sp.
19
-
 Z-dienelactone wild-type enzyme, pH 7.0, 24°C Pseudomonas sp.