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Literature summary for 3.1.1.45 extracted from

  • Perez-Pantoja, D.; Guzman, L.; Manzano, M.; Pieper, D.H.; Gonzalez, B.
    Role of tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II) gene modules in catabolism of 3-chlorobenzoate by Ralstonia eutropha JMP134(pJP4) (2000), Appl. Environ. Microbiol., 66, 1602-1608.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme encoded by gene tfdE in a module I of genes tfdC, tfdD, tfdE, and tfdF organized in one plasmid, functional expression in Ralstonia eutropha JMP222 and in Pseudomonas putida KT2442, 2 strains able to grow on 3-chlorobenzoate Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dienelactone + H2O Cupriavidus necator enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate maleylacetate
-
 ?
dienelactone + H2O Cupriavidus necator JMP 134-1 enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate maleylacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator JMP 134-1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.61
-
 crude enzyme extract from wild-type strain Ralstonia eutropha JMP134 Cupriavidus necator
1.96
-
 crude enzyme extract from recombinant strain Ralstonia eutropha JMP222 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-dienelactone + H2O
-
 Cupriavidus necator maleylacetate
-
 ?
cis-dienelactone + H2O
-
 Cupriavidus necator JMP 134-1 maleylacetate
-
 ?
dienelactone + H2O enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate Cupriavidus necator maleylacetate
-
 ?
dienelactone + H2O enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate Cupriavidus necator JMP 134-1 maleylacetate
-
 ?

Synonyms

Synonyms Comment Organism
dienelactone hydrolase
-
 Cupriavidus necator
DLH
-
 Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Cupriavidus necator