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Literature summary for 3.1.1.43 extracted from
- Substrate specificity of an alpha-amino acid ester hydrolase produced by Acetobacter turbidans A.T.C.C. 9325 (1974), Biochem. J., 137, 497-503.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ampicillin | hydrolysis, substrate inhibition | Acetobacter pasteurianus |  |
| ethyl D-alpha-aminophenylacetate | hydrolysis, substrate inhibition | Acetobacter pasteurianus | |
| methyl D-alpha-aminophenylacetate | hydrolysis, substrate inhibition | Acetobacter pasteurianus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
S-benzyl-L-cysteine ethyl ester | - |
Acetobacter pasteurianus | |
| 0.6 | - |
ampicillin | - |
Acetobacter pasteurianus | |
| 0.8 | - |
L-valine methyl ester | - |
Acetobacter pasteurianus | |
| 2.6 | - |
ethyl DL-alpha-amino-n-butyrate | - |
Acetobacter pasteurianus | |
| 4.3 | - |
methyl D-alpha-aminophenylacetate | - |
Acetobacter pasteurianus | |
| 5 | - |
glycine ethyl ester | - |
Acetobacter pasteurianus | |
| 24 | - |
methyl L-alpha-aminophenylacetate | - |
Acetobacter pasteurianus | |
| 32.2 | - |
methyl DL-alpha-aminoisobutyrate | - |
Acetobacter pasteurianus | |
| 40.9 | - |
D-alanine methyl ester | - |
Acetobacter pasteurianus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter pasteurianus | - |
ATCC 9325 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetobacter pasteurianus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 93.5 | - |
- |
Acetobacter pasteurianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-aminoisobutyric acid methyl ester + 7-amino-3-deacetoxycephalosporanic acid | - |
Acetobacter pasteurianus | methanol + ? | - |
? | |
| alpha-aminoisobutyric acid methyl ester + H2O | - |
Acetobacter pasteurianus | alpha-aminoisobutyric acid + methanol | - |
? | |
| D-alanine methyl ester + 7-amino-3-deacetoxycephalosporanic acid | - |
Acetobacter pasteurianus | methanol + ? | - |
? | |
| D-alanine methyl ester + H2O | - |
Acetobacter pasteurianus | D-alanine + methanol | - |
? | |
| D-alpha-phenylglycine methyl ester + 7-aminocephalosporanic acid | - |
Acetobacter pasteurianus | methanol + ? | - |
? | |
| D-alpha-phenylglycine methyl ester + H2O | - |
Acetobacter pasteurianus | D-alpha-phenylglycine + methanol | - |
? | |
| D-methionine methyl ester + 7-amino-3-deacetoxycephalosporanic acid | - |
Acetobacter pasteurianus | methanol + ? | - |
? | |
| D-methionine methyl ester + H2O | - |
Acetobacter pasteurianus | D-methionine + methanol | - |
? | |
| ethyl DL-alpha-amino-n-butyrate + H2O | - |
Acetobacter pasteurianus | ? | - |
? | |
| glycine ethyl ester + 7-aminocephalosporanic acid | - |
Acetobacter pasteurianus | ethanol + ? | - |
? | |
| glycine ethyl ester + H2O | - |
Acetobacter pasteurianus | glycine + ethanol | - |
? | |
| L-cysteine methyl ester + 7-amino-3-deacetoxycephalosporanic acid | - |
Acetobacter pasteurianus | methanol + ? | - |
? | |
| L-cysteine methyl ester + H2O | - |
Acetobacter pasteurianus | L-cysteine + methanol | - |
? | |
| L-serine methyl ester + 7-amino-3-deacetoxycephalosporanic acid | - |
Acetobacter pasteurianus | methanol + ? | - |
? | |
| L-serine methyl ester + H2O | - |
Acetobacter pasteurianus | L-serine + methanol | - |
? | |
| L-valine methyl ester + H2O | - |
Acetobacter pasteurianus | L-valine + methanol | - |
? | |
| S-benzyl-L-cysteine ethyl ester + H2O | - |
Acetobacter pasteurianus | S-benzyl-L-cysteine + ethanol | - |
? | |
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