Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethylene glycol | addition of ethylene glycol at 33 vol% to the reaction medium for cephalexin synthesis improves the yield from 70% to 95% | Acidovorax avenae |
Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli strain VKPM B-11246 | Acidovorax avenae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | synthesis of amino-betalactam antibiotic cephalexin by the recombinant enzyme produced in Escherichia coli VKPM B-11246 is more efficient in comparison with the wild-type enzyme prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915. Addition of ethylene glycol at 33 vol% to the reaction medium for cephalexin synthesis improves the yield from 70% to 95% | Acidovorax avenae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax avenae | - |
- |
- |
Acidovorax avenae VKPM B-9915 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme possesses high specificity to esters containing an amino group as a substituent on the Calpha atom of the acid, and it is capable to catalyze N-acylation of 7-aminocephem and 6-aminopenam by these esters with the formation of an amide bond. Synthesis of amino-beta-lactam antibiotic cephalexin from the key intermediate 7-aminodesacetoxycephalosporanic acid and the acylating agent of D-phenylglycine methyl ester by the recombinant enzyme in cell-free extract produced in Escherichia coli VKPM B-11246 is more efficient in comparison with the wild-type enzyme in cell-free extract prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915 | Acidovorax avenae | ? | - |
? | |
additional information | the enzyme possesses high specificity to esters containing an amino group as a substituent on the Calpha atom of the acid, and it is capable to catalyze N-acylation of 7-aminocephem and 6-aminopenam by these esters with the formation of an amide bond. Synthesis of amino-beta-lactam antibiotic cephalexin from the key intermediate 7-aminodesacetoxycephalosporanic acid and the acylating agent of D-phenylglycine methyl ester by the recombinant enzyme in cell-free extract produced in Escherichia coli VKPM B-11246 is more efficient in comparison with the wild-type enzyme in cell-free extract prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915 | Acidovorax avenae VKPM B-9915 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AEH | - |
Acidovorax avenae |
alpha-amino acid ester hydrolase | - |
Acidovorax avenae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Acidovorax avenae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.25 | assay at | Acidovorax avenae |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the class of hydrolases with alpha/beta-type folding | Acidovorax avenae |