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Literature summary for 3.1.1.42 extracted from

  • Benoit, I.; Asther, M.; Bourne, Y.; Navarro, D.; Canaan, S.; Lesage-Meessen, L.; Herweijer, M.; Coutinho, P.M.; Asther, M.; Record, E.
    Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger (2007), Appl. Environ. Microbiol., 73, 5624-5632.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
PCR Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5
-
 chlorogenic acid
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 determined by gel filtration and alternatively by mass spectrometry with matrix assisted laser desorption and ionization time-of-flight mass spectrometers Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A7YN26
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorogenic acid + H2O
-
 Aspergillus niger ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Aspergillus niger

Synonyms

Synonyms Comment Organism
chlorogenic acid hydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.8 7.8 stable for 90 min Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger
-
-
 4.6