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Literature summary for 3.1.1.42 extracted from

  • Asther, M.; Estrada Alvarado, M.I.; Haon, M.; Navarro, D.; Asther, M.; Lesage-Meessen, L.; Record, E.
    Purification and characterization of a chlorogenic acid hydrolase from Aspergillus niger catalyzing the hydrolysis of chlorogenic acid (2005), J. Biotechnol., 115, 47-56.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
chlorogenic acid 37°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
2 * 80000, SDS-PAGE Aspergillus niger
170000
-
gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger BRFM 131
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2
-
37°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorogenic acid + H2O hydrolysis of 86% or 100% of chlorogenic acid in apple marc and autoclaved coffee pulp, resp. Aspergillus niger caffeate + quinate
-
?
chlorogenic acid + H2O hydrolysis of 86% or 100% of chlorogenic acid in apple marc and autoclaved coffee pulp, resp. Aspergillus niger BRFM 131 caffeate + quinate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Aspergillus niger
45
-
10 min, 27% loss of activity Aspergillus niger
50
-
10 min, 84% loss of activity Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22
-
chlorogenic acid 37°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7 stable within this range Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger isoelectric focusing
-
6