KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
chlorogenic acid | 37°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
2 * 80000, SDS-PAGE | Aspergillus niger |
170000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger BRFM 131 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.2 | - |
37°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorogenic acid + H2O | hydrolysis of 86% or 100% of chlorogenic acid in apple marc and autoclaved coffee pulp, resp. | Aspergillus niger | caffeate + quinate | - |
? | |
chlorogenic acid + H2O | hydrolysis of 86% or 100% of chlorogenic acid in apple marc and autoclaved coffee pulp, resp. | Aspergillus niger BRFM 131 | caffeate + quinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Aspergillus niger |
45 | - |
10 min, 27% loss of activity | Aspergillus niger |
50 | - |
10 min, 84% loss of activity | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
chlorogenic acid | 37°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | stable within this range | Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | isoelectric focusing | - |
6 |