Literature summary for 3.1.1.41 extracted from

Tao, W.; Xu, Q.; Huang, H.; Li, S.
Efficient production of peracetic acid in aqueous solution with cephalosporin-deacetylating acetyl xylan esterase from Bacillus subtilis (2015), Process Biochem., 50, 2121-2127 .

No PubMed abstract available

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
synthesis	synthesis of peracetic acid. Recombinant enzyme can be efficiently produced in a low-cost autoinduction medium with an activity of 6800 U/ml. Optimal synthesis of peracetic is achieved with 0.30 mg/ml crude enzyme, 300 mM glycerol triacetate, and 1 M hydrogen peroxide, pH 8.0, and 2°C, which produces approximately 150 mM of peracetic acid within 5 min. When immobilized on an acrylate amino resin, the activity of the immobilized enzyme is 383.7 U/g	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
ethanol	product inhibition	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1	-	H2O2	free enzyme, pH 6.5, 25°C	Bacillus subtilis
1.15	-	H2O2	immobilized enzyme, pH 6.5, 25°C	Bacillus subtilis
140.7	-	glycerol triacetate	free enzyme, pH 6.5, 25°C	Bacillus subtilis
315.3	-	glycerol triacetate	immobilized enzyme, pH 6.5, 25°C	Bacillus subtilis
409.1	-	ethyl acetate	free enzyme, pH 6.5, 25°C	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	M1JUH6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ethyl acetate + H2O2	-	Bacillus subtilis	ethanol + peracetic acid	-	?
glycerol triacetate + H2O2	-	Bacillus subtilis	glycerol diacetate + peracetic acid	-	?

Subunits

Subunits	Comment	Organism
?	x * 35600, SDS-PAGE	Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.089	-	ethyl acetate	free enzyme, pH 6.5, 25°C	Bacillus subtilis
0.4	-	glycerol triacetate	immobilized enzyme, pH 6.5, 25°C	Bacillus subtilis
1.09	-	H2O2	immobilized enzyme, pH 6.5, 25°C	Bacillus subtilis
2.53	-	glycerol triacetate	free enzyme, pH 6.5, 25°C	Bacillus subtilis
3.3	-	H2O2	free enzyme, pH 6.5, 25°C	Bacillus subtilis

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Release 2023.1 (January 2023)