Application | Comment | Organism |
---|---|---|
synthesis | enzyme reaction products deacetyl cephalosporins are a highly valuable starting material for producing semisynthetic beta-lactam antibiotics | Bacillus pumilus |
Cloned (Comment) | Organism |
---|---|
gene axe, expression of His-tagged enzyme in Escherichia coli | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bacillus pumilus | |
215 | - |
cephalosporin C | pH 7.3, 25°C, recombinant enzyme | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
gene axe | - |
Bacillus pumilus CECT 5072 | - |
gene axe | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-aminocephalosporanic acid + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus | 7-amino-3-deacetylcephalosporanic acid + acetate | - |
? | |
7-aminocephalosporanic acid + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus CECT 5072 | 7-amino-3-deacetylcephalosporanic acid + acetate | - |
? | |
cephalosporin C + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus | deacetylcephalosporin C + acetate | - |
? | |
cephalosporin C + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus CECT 5072 | deacetylcephalosporin C + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetyl xylan esterase | - |
Bacillus pumilus |
AXE | - |
Bacillus pumilus |
cephalosporin C acetyl esterase | - |
Bacillus pumilus |
cephalosporin C deacetylase | - |
Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
enzymatic hydrolysis around pH 7.0 is the most effective method for preparing deacetyl derivatives of cephalosporin | Bacillus pumilus |