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Literature summary for 3.1.1.41 extracted from
- Purification and characterization of a cephalosporin esterase from Phodosporidium toruloides (1997), Appl. Environ. Microbiol., 63, 4807-4811.
General Stability
| General Stability | Organism |
|---|---|
| unstable after deglycosylation with endoglycosidase H | Rhodotorula toruloides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4-dichloroisocoumarin | - |
Rhodotorula toruloides |  |
| dimethyl phosphite | - |
Rhodotorula toruloides | |
| PMSF | - |
Rhodotorula toruloides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 51.8 | - |
cephalosporin C | - |
Rhodotorula toruloides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61315 | - |
x * 61315, calculation from amino acid sequence | Rhodotorula toruloides |
| 80000 | - |
x * 80000, SDS-PAGE | Rhodotorula toruloides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Rhodotorula toruloides | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein, sugar moieties may play a role in stabilizing the native structure of the enzyme | Rhodotorula toruloides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodotorula toruloides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhodotorula toruloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cephalosporin C + H2O | - |
Bacillus subtilis | deacetylcephalosporin C + acetate | - |
? | |
| cephalosporin C + H2O | - |
Rhodotorula toruloides | deacetylcephalosporin C + acetate | - |
? | |
| deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate | - |
Bacillus subtilis | 7-aminocephalosporanic acid + ? | - |
? | |
| deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate | - |
Rhodotorula toruloides | 7-aminocephalosporanic acid + ? | - |
? | |
| deacetyl-7-aminocephalosporanic acid + isopropenyl acetate | - |
Bacillus subtilis | 7-aminocephalosporanic acid + ? | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Rhodotorula toruloides | 4-nitrophenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE | Rhodotorula toruloides |
| ? | x * 61315, calculation from amino acid sequence | Rhodotorula toruloides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Rhodotorula toruloides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Bacillus subtilis |
| 25 | - |
unstable above | Rhodotorula toruloides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Rhodotorula toruloides |
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