BRENDA - Enzyme Database
show all sequences of 3.1.1.40

Studies on lichen enzymes. Purification and properties of an orsellinate depside hydrolase obtained from Lasallia pustulata

Schultz, J.; Mosbach, K.; Eur. J. Biochem. 22, 153-157 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
diisopropylfluorophosphate
-
Lasallia pustulata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.056
-
Lecanoric acid
-
Lasallia pustulata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration, ultracentrifugation, SDS-PAGE
Lasallia pustulata
Organism
Organism
UniProt
Commentary
Textmining
Lasallia pustulata
-
lichen
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Lasallia pustulata
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1300
-
-
Lasallia pustulata
Storage Stability
Storage Stability
Organism
frozen, 6 months, no loss of activity
Lasallia pustulata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
erythrin + H2O
-
80929
Lasallia pustulata
?
-
-
-
?
evernic acid + H2O
-
80929
Lasallia pustulata
orsellinic acid + everninic acid
-
80929
Lasallia pustulata
?
gyrophoric acid + H2O
-
80929
Lasallia pustulata
orsellinic acid
-
80929
Lasallia pustulata
?
lecanoric acid + H2O
i.e. orsellinate depside
80929
Lasallia pustulata
orsellinic acid
-
80929
Lasallia pustulata
?
methyllecanorate + H2O
-
80929
Lasallia pustulata
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 42000, SDS-PAGE
Lasallia pustulata
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
stable for 4 days
Lasallia pustulata
57
-
60 min stable, loss of activity if incubated longer
Lasallia pustulata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diisopropylfluorophosphate
-
Lasallia pustulata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.056
-
Lecanoric acid
-
Lasallia pustulata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration, ultracentrifugation, SDS-PAGE
Lasallia pustulata
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lasallia pustulata
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1300
-
-
Lasallia pustulata
Storage Stability (protein specific)
Storage Stability
Organism
frozen, 6 months, no loss of activity
Lasallia pustulata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
erythrin + H2O
-
80929
Lasallia pustulata
?
-
-
-
?
evernic acid + H2O
-
80929
Lasallia pustulata
orsellinic acid + everninic acid
-
80929
Lasallia pustulata
?
gyrophoric acid + H2O
-
80929
Lasallia pustulata
orsellinic acid
-
80929
Lasallia pustulata
?
lecanoric acid + H2O
i.e. orsellinate depside
80929
Lasallia pustulata
orsellinic acid
-
80929
Lasallia pustulata
?
methyllecanorate + H2O
-
80929
Lasallia pustulata
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 42000, SDS-PAGE
Lasallia pustulata
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
stable for 4 days
Lasallia pustulata
57
-
60 min stable, loss of activity if incubated longer
Lasallia pustulata
Other publictions for EC 3.1.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
80930
Garcia-Junceda
-
Kinetics and stability of an i ...
Evernia prunastri
Enzyme Microb. Technol.
13
275-279
1991
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1
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-
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1
1
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-
-
-
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1
-
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1
-
-
-
-
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2
-
-
-
-
2
-
1
-
-
-
-
-
-
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1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
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2
-
1
-
-
-
-
-
-
-
-
-
80929
Schultz
Studies on lichen enzymes. Pur ...
Lasallia pustulata
Eur. J. Biochem.
22
153-157
1971
-
-
-
-
-
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1
1
-
-
1
-
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5
-
-
1
-
-
-
1
1
5
1
-
-
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2
-
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-
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1
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1
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1
-
-
-
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1
-
-
1
1
5
1
-
-
2
-
-
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-
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