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Literature summary for 3.1.1.4 extracted from
- Streptomyces violaceoruber phospholipase A2 expression in Pichia pastoris, properties, and application in oil degumming (2015), Appl. Biochem. Biotechnol., 175, 3195-3206 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | application in oil degumming. In the enzymatic degumming process, the phosphorus content is reduced to 20.74 mg/kg within 6 h, which is promising for the industrial application | Streptomyces violaceoruber |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris GS115 under the control of AOX1 promoter | Streptomyces violaceoruber |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activity increases from 0.2 mM up to 1.0 mM Ca2+. The structure of the calcium-bound domain is distinct from that without the ion | Streptomyces violaceoruber |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces violaceoruber | Q6UV28 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme expressed in Pichia pastoris is post-translational modified, while the enzyme secreted by prokaryotes does not occur glycosylation | Streptomyces violaceoruber |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces violaceoruber |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospholipase A2 | - |
Streptomyces violaceoruber |
| PLA2 | - |
Streptomyces violaceoruber |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme expressed in Pichia pastoris | Streptomyces violaceoruber |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 65 | 35°C: about 70% of maximal activity, 65°C: about 70% of maximal activity, enzyme expressed in Pichia pastoris | Streptomyces violaceoruber |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | 1 h, pH 6, stable, recombinant enzyme | Streptomyces violaceoruber |
| 35 | - |
1 h, pH 6, about 35% loss of activity, recombinant enzyme | Streptomyces violaceoruber |
| 45 | - |
1 h, pH 6, 50% loss of activity, recombinant enzyme | Streptomyces violaceoruber |
| 50 | - |
1 h, pH 6, 80% loss of activity, recombinant enzyme | Streptomyces violaceoruber |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Streptomyces violaceoruber |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | at 50°C, pH 5.5: about 75% of maximal activity, pH 7.0: about 65% of maximal activity, recombinant enzyme | Streptomyces violaceoruber |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.2 | at 20°C, 5 h, the recombinant enzyme is stable at a pH range of 4.5-6.2 | Streptomyces violaceoruber |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces violaceoruber | enzyme expressed in Pichia pastoris, isoelectric focusing | - |
5.9 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Streptomyces violaceoruber | high concentration of Ca2+ is necessary for the expression of the enzyme activity | up |
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