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Literature summary for 3.1.1.4 extracted from

  • Nunes, D.C.; Rodrigues, R.S.; Lucena, M.N.; Cologna, C.T.; Oliveira, A.C.; Hamaguchi, A.; Homsi-Brandeburgo, M.I.; Arantes, E.C.; Teixeira, D.N.; Ueira-Vieira, C.; Rodrigues, V.M.
    Isolation and functional characterization of proinflammatory acidic phospholipase A2 from Bothrops leucurus snake venom (2011), Comp. Biochem. Physiol. C, 154, 226-233.
    View publication on PubMed
Search for more literature for 3.1.1.4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 x * 15000, SDS-PAGE Bothrops leucurus

Organism

Organism UniProt Comment Textmining
Bothrops leucurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose column chromatography and phenyl-Sepharose column chromatography Bothrops leucurus

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Bothrops leucurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69.3
-
 enzyme from crude venom, pH and temperature not specified in the publication Bothrops leucurus
159.9
-
 purified enzyme, pH and temperature not specified in the publication Bothrops leucurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has hemolytic activity Bothrops leucurus ?
-
 ?
phosphatidylcholine + H2O
-
 Bothrops leucurus 1-acylglycerophosphocholine + fatty acid
-
 ?

Subunits

Subunits Comment Organism
? x * 15000, SDS-PAGE Bothrops leucurus

Synonyms

Synonyms Comment Organism
acidic phospholipase A2
-
 Bothrops leucurus
PLA2
-
 Bothrops leucurus

pI Value

Organism Comment pI Value Maximum pI Value
Bothrops leucurus isoelectric focusing
-
 5.4

General Information

General Information Comment Organism
physiological function the enzyme does not show myotoxic activity, but induces edema and actively participates in the pathogenesis of snake bite. The enzyme is able to induce increments in interleukin-12p40, TNF-alpha, interleukin-1beta and interleukin-6 levels and no variation of interleukin-8 and interleukin-10 in human peripheral blood mononuclear cells stimulated in vitro, suggesting that the enzyme induces proinflammatory cytokine production by human mononuclear cells Bothrops leucurus