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Literature summary for 3.1.1.4 extracted from
- Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli (2009), Protein Expr. Purif., 67, 82-87.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | simple and inexpensive protein expression, refolding and purification system may be useful for site-directed mutagenesis experiments of sPLA2-IID which will advance the understanding of the structurefunction relationship and biological effects of the protein | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA-coding sequence subcloned into the vector pET3a, and expressed as inclusion bodies in Escherichia coli strain BL21(DE3)[pLysS] | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | loss of hydrolytic activity | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | shows Ca2+-dependent hydrolytic activity | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by single-step cation-exchange chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 63 | - |
in 20 mM HEPES buffer, 20 mM NaCl, 1 mM CaCl2, pH and temperature not specified in the publication | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 5 mM Tris-HCl buffer, pH 7.5 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | unilamellar liposomes composed of a 9:1 M ratio of dioleoyl phosphatidylcholine and dioleoyl phosphatidylglycerol, release of fatty acid | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| secreted group IID phospholipase A2 | - |
Homo sapiens |
| sPLA2-IID | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | is involved in the regulation of immune and inflammatory responses | Homo sapiens |
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Release 2023.1 (January 2023)
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