Literature summary for 3.1.1.4 extracted from

Chiou, Y.; Cheng, Y.; Kao, P.; Wang, J.; Chang, L.
Mutations on the N-terminal region abolish differentially the enzymatic activity, membrane-damaging activity and cytotoxicity of Taiwan cobra phospholipase A2 (2008), Toxicon, 51, 270-279.

Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis	Naja atra

Protein Variants

Protein Variants	Comment	Organism
additional information	removal of N-terminal heptapeptide causes a complete loss of membrane-damaging activity, whilst the mutants with an extra Met before Asn1 or substituting Asn1 with Met still retain approximately 40.9% and 82.9% membrane-damaging activity of the native enzyme, respectively. Mutations on the N-terminal region do not greatly affect the Ca2+-binding ability but cause a precipitous drop in PLA2 activity. The gross conformation of the mutants is altered compared to the native enzyme, overview. The enzymatic activity of mutants M-PLA2, PLA2(N1M) and PLA2(DELTAN7) are approximately 3.6%, 5.3% and 0.6% of that of native PLA2	Naja atra
N1M	site-directed mutagenesis, the mutants with an extra Met before Asn1 or substituting Asn1 with Met still retain approximately 40.9% and 82.9% membrane-damaging activity of the native enzyme, respectively. Mutations on the N-terminal region do not greatly affect the Ca2+-binding ability but cause a precipitous drop in PLA2 activity. The gross conformation of the mutant is altered compared to the native enzyme, overview. The enzymatic activity of the mutant is approximately 5.3% of that of native PLA2	Naja atra

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Naja atra	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Naja atra

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Naja atra	native and mutated PLA2s induce apoptotic death of U937 cells	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Naja atra	P00598	Taiwan cobra	-

Renatured (Commentary)

Renatured (Comment)	Organism
native PLA2 is labeled with NTSB and then refolded according to the same procedure for refolding of mutated PLA2. The refolded PLA2 exhibited enzymatic activity and electrophoretic mobility indistinguishable from those of native PLA2	Naja atra

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Naja atra	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	native and mutated PLA2s induce apoptotic death of U937 cells	Naja atra	?	-	?

Subunits

Subunits	Comment	Organism
More	analysis of the secondary structure of wild-type and N-terminal mutant enzymes, the structures are dominated by alpha-helical structure, overview	Naja atra

Synonyms

Synonyms	Comment	Organism
PLA2	-	Naja atra

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Naja atra

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Naja atra

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Release 2023.1 (January 2023)