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Literature summary for 3.1.1.4 extracted from

  • Jovel, S.R.; Kumagai, T.; Danshiitsoodol, N.; Matoba, Y.; Nishimura, M.; Sugiyama, M.
    Purification and characterization of the second Streptomyces phospholipase A2 refolded from an inclusion body (2006), Protein Expr. Purif., 50, 82-88.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
secreted phospholipase A2, DNA and amino acid sequence determination and analysis Streptomyces violaceoruber
secreted phospholipase A2, DNA and amino acid sequence determination and analysis, expression of in Escherichia coli strain BL21(DE3) in inclusion bodies Streptomyces coelicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted phospholipase A2 Streptomyces coelicolor
-
-
extracellular secreted phospholipase A2 Streptomyces violaceoruber
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
recombinant secreted phospholipase A2, gel filtration Streptomyces coelicolor
13000
-
1 * 13000, recombinant secreted phospholipase A2, SDS-PAGE, 1 * 13530, secreted phospholipase A2, MALDI-TOF mass spectrometry Streptomyces coelicolor
13530
-
1 * 13000, recombinant secreted phospholipase A2, SDS-PAGE, 1 * 13530, secreted phospholipase A2, MALDI-TOF mass spectrometry Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-
Streptomyces violaceoruber Q6UV28
-
-
Streptomyces violaceoruber A-2688 Q6UV28
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant solubilized secreted phospholipase A2 to homogeneity Streptomyces coelicolor

Renatured (Commentary)

Renatured (Comment) Organism
recombinant secreted PLA2 from inclusion bodies, after expression in Escherichia coli strain BL21(DE3), by solubilization with sodium deoxycholate and 2 M urea at pH 12, followed by refolding through dilution, overview Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alpha-phosphatidylcholine + H2O
-
Streptomyces coelicolor 1-acylglycerophosphocholine + a carboxylate
-
?
L-alpha-phosphatidylcholine + H2O
-
Streptomyces coelicolor A3(2) 1-acylglycerophosphocholine + a carboxylate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 13000, recombinant secreted phospholipase A2, SDS-PAGE, 1 * 13530, secreted phospholipase A2, MALDI-TOF mass spectrometry Streptomyces coelicolor
More three-dimensional structure and homology modeling, overview Streptomyces coelicolor
More three-dimensional structure and homology modeling, overview Streptomyces violaceoruber

Synonyms

Synonyms Comment Organism
PLA2
-
Streptomyces coelicolor
PLA2
-
Streptomyces violaceoruber
SCO1048 protein
-
Streptomyces coelicolor
secreted phospholipase A2
-
Streptomyces coelicolor
secreted phospholipase A2
-
Streptomyces violaceoruber

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptomyces coelicolor
37
-
assay at Streptomyces violaceoruber

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant secreted PLA2, stable for 10 min at pH 8.0 in the presence of 50 mM CaCl2 Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Streptomyces violaceoruber
8
-
-
Streptomyces coelicolor

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.3
-
recombinant secreted PLA2, stable at pH 6.3 and above, but unstable at pH 4.1 or pH 5.1 Streptomyces coelicolor

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces coelicolor isoelectric focusing, secreted PLA2
-
8.6