Cloned (Comment) | Organism |
---|---|
secreted phospholipase A2, DNA and amino acid sequence determination and analysis | Streptomyces violaceoruber |
secreted phospholipase A2, DNA and amino acid sequence determination and analysis, expression of in Escherichia coli strain BL21(DE3) in inclusion bodies | Streptomyces coelicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted phospholipase A2 | Streptomyces coelicolor | - |
- |
extracellular | secreted phospholipase A2 | Streptomyces violaceoruber | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11000 | - |
recombinant secreted phospholipase A2, gel filtration | Streptomyces coelicolor |
13000 | - |
1 * 13000, recombinant secreted phospholipase A2, SDS-PAGE, 1 * 13530, secreted phospholipase A2, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
13530 | - |
1 * 13000, recombinant secreted phospholipase A2, SDS-PAGE, 1 * 13530, secreted phospholipase A2, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Streptomyces violaceoruber | Q6UV28 | - |
- |
Streptomyces violaceoruber A-2688 | Q6UV28 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant solubilized secreted phospholipase A2 to homogeneity | Streptomyces coelicolor |
Renatured (Comment) | Organism |
---|---|
recombinant secreted PLA2 from inclusion bodies, after expression in Escherichia coli strain BL21(DE3), by solubilization with sodium deoxycholate and 2 M urea at pH 12, followed by refolding through dilution, overview | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-phosphatidylcholine + H2O | - |
Streptomyces coelicolor | 1-acylglycerophosphocholine + a carboxylate | - |
? | |
L-alpha-phosphatidylcholine + H2O | - |
Streptomyces coelicolor A3(2) | 1-acylglycerophosphocholine + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 13000, recombinant secreted phospholipase A2, SDS-PAGE, 1 * 13530, secreted phospholipase A2, MALDI-TOF mass spectrometry | Streptomyces coelicolor |
More | three-dimensional structure and homology modeling, overview | Streptomyces coelicolor |
More | three-dimensional structure and homology modeling, overview | Streptomyces violaceoruber |
Synonyms | Comment | Organism |
---|---|---|
PLA2 | - |
Streptomyces coelicolor |
PLA2 | - |
Streptomyces violaceoruber |
SCO1048 protein | - |
Streptomyces coelicolor |
secreted phospholipase A2 | - |
Streptomyces coelicolor |
secreted phospholipase A2 | - |
Streptomyces violaceoruber |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces coelicolor |
37 | - |
assay at | Streptomyces violaceoruber |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant secreted PLA2, stable for 10 min at pH 8.0 in the presence of 50 mM CaCl2 | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Streptomyces violaceoruber |
8 | - |
- |
Streptomyces coelicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
recombinant secreted PLA2, stable at pH 6.3 and above, but unstable at pH 4.1 or pH 5.1 | Streptomyces coelicolor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces coelicolor | isoelectric focusing, secreted PLA2 | - |
8.6 |