Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F5W | site-directed mutagenesis, mutation of the substrate binding pocket leads to reduced enzyme activity, the inhibitory effect of the F5W mutation results from the high affinity of Trp for the membrane-water interface | Homo sapiens |
V3W | site-directed mutagenesis, mutation of the membrane binding surface residue results in substantial enhancement of the enzyme activity, the stimulatory effect of the V3W mutation results from the high affinity of Trp for the membrane-water interface | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and membrane binding of recombinant wild-type and mutant enzymes, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretory phospholipase A2 | Homo sapiens | - |
- |
membrane | hIBPLA2, structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2, overview | Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant solubilized wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heparin affinity chromatography and gel filtration to homogeneity | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli inclusion bodies | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-bis-(10-pyrenedecanoyl)-sn-glycero-3-phosphocholine + H2O | - |
Homo sapiens | 1-(10-pyrenedecanoyl)-glycero-3-phosphocholine + 10-pyrenedecanoate | - |
? | |
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Homo sapiens | 1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate | - |
? | |
1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol + H2O | - |
Homo sapiens | 1-palmitoyl-glycero-3-phosphoglycerol + palmitate | - |
? | |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O | - |
Homo sapiens | 1-palmitoyl-sn-glycero-3-phosphocholine + oleate | - |
? | |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol + H2O | - |
Homo sapiens | 1-palmitoyl-sn-glycero-3-phosphorylglycerol + oleate | - |
? | |
1-palmitoyl-2-stearoyl-dibromo-sn-glycero-3-phosphocholine + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hIIAPLA2 | - |
Homo sapiens |
human group IIA phospholipase A2 | - |
Homo sapiens |
PLA2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |