Crystallization (Comment) | Organism |
---|---|
purified crotoxin, 10 mg/ml protein in 20 mM TrisHCl buffer, pH 8.0, hanging-drop vapour-diffusion method, 0.001 ml of protein and of reservoir solution are mixed and equilibrated against 1 ml of reservoir solution containing 0.1 M Tris-HCl pH 8.5 and 8% w/v PEG 8000 within 17 days, or of reservoir solution containing 0.05 M cadmium sulfate, 0.1 M HEPES, pH 7.5, and 1 M sodium acetate within within 30 days, or of reservoir solution containing 28% v/v PEG 400, 0.1 M HEPES pH 7.5, 0.2 M calcium chloride dehydrate and 3-9% v/v glycerol, X-ray diffraction structure determination and analysis at 1.75-2.6 A resolution | Crotalus durissus terrificus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9490 | - |
1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit | Crotalus durissus terrificus |
14350 | - |
1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit | Crotalus durissus terrificus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Crotalus durissus terrificus | crotoxin, a potent neurotoxin from the venom of the Brazilian rattlesnake, exists as a heterodimer formed between a phospholipase A2 and a catalytically inactive acidic phospholipase A2 analogue crotapotin, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crotalus durissus terrificus | - |
South American rattlesnake | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Crotalus durissus terrificus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | crotoxin, a potent neurotoxin from the venom of the Brazilian rattlesnake, exists as a heterodimer formed between a phospholipase A2 and a catalytically inactive acidic phospholipase A2 analogue crotapotin, overview | Crotalus durissus terrificus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit | Crotalus durissus terrificus |
More | crotoxin, a potent neurotoxin from the venom of the South American rattlesnake, exists as a heterodimer formed between a phospholipase A2 and a catalytically inactive acidic phospholipase A2 analogue crotapotin, crotapotin consists of three polypeptide chains linked together by disulfide bridges and acts as an inhibitory chaperone subunit that prevents promiscuous interactions of the phospholipase with phospholipids on membrane surfaces other than its target membrane, overview | Crotalus durissus terrificus |
Synonyms | Comment | Organism |
---|---|---|
crotoxin | - |
Crotalus durissus terrificus |
PLA2 | - |
Crotalus durissus terrificus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Crotalus durissus terrificus | crotapin subunit, sequence calculation | - |
3.4 |
Crotalus durissus terrificus | phospholipase A2 subunit, sequence calculation | - |
8.2 |