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Literature summary for 3.1.1.4 extracted from

  • Wang, B.; Lu, D.; Gao, R.; Yang, Z.; Cao, S.; Feng, Y.
    A novel phospholipase A2/esterase from hyperthermophilic archaeon Aeropyrum pernix K1 (2004), Protein Expr. Purif., 35, 199-205.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 1 mM, residual activity 82.1% Aeropyrum pernix
KCl 1 mM, residual activity 78.9% Aeropyrum pernix
MgCl2 1 mM, residual activity 71.6% Aeropyrum pernix
additional information not inhibitory: EDTA Aeropyrum pernix
NaCl 1 mM, residual activity 58.5% Aeropyrum pernix
ZnCl2 1 mM, no residual activity Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.103
-
p-nitrophenyl propionate 90°C, pH 8.0 Aeropyrum pernix

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for Ca2+ Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
x * 18000, SDS-PAGE Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
var. K1, bifunctional enzyme bwith both phospholipase A2 and esterase activity
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
249
-
90°C, pH 8.0 Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: p-nitrophenyl palmitate, p-nitrophenyl stearate Aeropyrum pernix ?
-
?
p-nitrophenyl laurate + H2O 23.7% of the activity with p-nitrophenyl propionate Aeropyrum pernix p-nitrophenol + laurate
-
?
p-nitrophenyl octanoate + H2O 79.5% of the activity with p-nitrophenyl propionate Aeropyrum pernix p-nitrophenol + octanoic acid
-
?
p-nitrophenyl propionate + H2O
-
Aeropyrum pernix p-nitrophenol + propionate
-
?

Subunits

Subunits Comment Organism
? x * 18000, SDS-PAGE Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
half-life of about 1 h Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39
-
p-nitrophenyl propionate 90°C, pH 8.0 Aeropyrum pernix