Crystallization (Comment) | Organism |
---|---|
- |
Crotalus adamanteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
Ba2+ | - |
Crotalus adamanteus | |
Ba2+ | - |
Rattus norvegicus | |
Ba2+ | - |
Sus scrofa | |
Cd2+ | - |
Sus scrofa | |
Co2+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
diisopropyl fluorophosphate | - |
Crotalus atrox | |
EDTA | - |
Rattus norvegicus | |
EDTA | - |
Sus scrofa | |
Fe2+ | - |
Crotalus adamanteus | |
Fe2+ | - |
Rattus norvegicus | |
Pb2+ | - |
Sus scrofa | |
Zn2+ | - |
Crotalus adamanteus | |
Zn2+ | - |
Crotalus atrox | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
lecithin | in ether | Crotalus atrox | |
8.5 | - |
lecithin | in chloroform | Crotalus atrox |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus | |
Ca2+ | - |
Sus scrofa | |
Ca2+ | - |
Crotalus adamanteus | |
Ca2+ | - |
Crotalus atrox | |
Cd2+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
Mg2+ | can replace Ca2+, decreased activity | Crotalus adamanteus | |
Mn2+ | can replace Ca2+ but enzyme shows decreased activity | Crotalus adamanteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crotalus adamanteus | - |
- |
- |
Crotalus atrox | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, column chromatography | Crotalus atrox |
column chromatography | Sus scrofa |
gel filtration, column chromatography | Crotalus adamanteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Sus scrofa | - |
venom | - |
Crotalus adamanteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3150 | - |
substrate: phosphatidylcholine | Crotalus adamanteus |
3250 | - |
substrate: phosphatidylcholine | Crotalus adamanteus |
Storage Stability | Organism |
---|---|
4°C, for months without loss of activity | Crotalus adamanteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphatidylglycerol + H2O | - |
Rattus norvegicus | ? | - |
? | |
phosphatidic acid + H2O | - |
Sus scrofa | 1-acylglycerophosphate + fatty acid | - |
? | |
phosphatidic acid + H2O | - |
Crotalus adamanteus | 1-acylglycerophosphate + fatty acid | - |
? | |
phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-acylglycerophosphocholine + fatty acid | - |
? | |
phosphatidylcholine + H2O | - |
Sus scrofa | 1-acylglycerophosphocholine + fatty acid | - |
? | |
phosphatidylcholine + H2O | - |
Crotalus adamanteus | 1-acylglycerophosphocholine + fatty acid | - |
? | |
phosphatidylcholine + H2O | - |
Crotalus atrox | 1-acylglycerophosphocholine + fatty acid | - |
? | |
phosphatidylethanolamine + H2O | - |
Rattus norvegicus | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
phosphatidylethanolamine + H2O | - |
Sus scrofa | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
phosphatidylglycerol + H2O | - |
Rattus norvegicus | 1-acylglycerol + fatty acid | - |
? | |
phosphatidylglycerol + H2O | - |
Sus scrofa | 1-acylglycerol + fatty acid | - |
? | |
phosphatidylglycerol + H2O | - |
Crotalus adamanteus | 1-acylglycerol + fatty acid | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
for 30 min., pH 3.0 | Crotalus atrox |
98 | - |
for 5 min., pH 4.0, no loss of activity, for 1 h, pH 4.0, 45% of activity are recovered | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.7 | - |
phosphatidylcholine | - |
Crotalus adamanteus |