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Literature summary for 3.1.1.4 extracted from
- Phospholipase A2 from Trypanosoma congolense: characterization and haematological properties (1993), Cell Biochem. Funct., 11, 125-130.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Trypanosoma congolense |  |
| p-hydroxymercuribenzoate | - |
Trypanosoma congolense | |
| tributyltin oxide | - |
Trypanosoma congolense | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.85 | - |
dimyristoyl-sn-phosphatidylcholine | - |
Trypanosoma congolense | |
| 2.12 | - |
dioleolyl-sn-phosphatidylcholine | - |
Trypanosoma congolense | |
| 3.65 | - |
dimyristoyl-sn-phosphatidylserine | - |
Trypanosoma congolense | |
| 4.6 | - |
dioleolyl-sn-phosphatidylethanolamine | - |
Trypanosoma congolense | |
| 56 | - |
dipalmitoyl-sn-phosphatidylserine | - |
Trypanosoma congolense | |
| 58 | - |
dimyristoyl-sn-phosphatidylinositol | - |
Trypanosoma congolense | |
| 86 | - |
dipalmitoyl-phosphatidylcholine | - |
Trypanosoma congolense | |
| 98 | - |
dipalmitoyl-phosphatidylethanolamine | - |
Trypanosoma congolense | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Trypanosoma congolense | |
| Zn2+ | - |
Trypanosoma congolense | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16500 | - |
1 * 16500 + 1 * 18000, SDS-PAGE | Trypanosoma congolense |
| 18000 | - |
1 * 16500 + 1 * 18000, SDS-PAGE | Trypanosoma congolense |
| 34500 | - |
dimer with a 16500 and a 18000 subunit, SDS-PAGE | Trypanosoma congolense |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Trypanosoma congolense | hemolytic activity of basic PLA2 | ? | - |
? | |
| phospholipids + H2O | Trypanosoma congolense | influence on platelet aggregation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma congolense | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange chromatography | Trypanosoma congolense |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, high stability even at low concentrations, i.e. 50 mg,in 20 mM phosphate buffer pH 6.8 containing 2 mM of 2-mercaptoethanol | Trypanosoma congolense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dimyristoyl-sn-phosphatidylcholine + H2O | - |
Trypanosoma congolense | 1-myristoyl-phosphorylcholine + myristic acid | - |
? | |
| 1,2-dimyristoyl-sn-phosphatidylinositol + H2O | - |
Trypanosoma congolense | 1-myristoyl-sn-phosphatidylinositol + myristic acid | - |
? | |
| 1,2-dimyristoyl-sn-phosphatidylserine + H2O | - |
Trypanosoma congolense | 1-myristoyl-sn-phosphatidylserine + myristic acid | - |
? | |
| 1,2-dioleoyl-sn-phosphatidylcholine + H2O | - |
Trypanosoma congolense | 1-oleoyl-sn-phosphatidylcholine + oleate | - |
? | |
| 1,2-dioleoyl-sn-phosphatidylethanolamine + H2O | - |
Trypanosoma congolense | 1-oleoyl-sn-phosphatidylethanolamine + oleic acid | - |
? | |
| 1,2-dipalmitoyl-phosphatidylcholine + H2O | - |
Trypanosoma congolense | 1-palmitoyl-glycerophosphorylcholine + palmitic acid | - |
? | |
| 1,2-dipalmitoyl-phosphatidylethanolamine + H2O | - |
Trypanosoma congolense | 1-palmitoyl-phosphatidylethanolamine + palmitic acid | - |
? | |
| 1,2-dipalmitoyl-sn-phosphatidylserine + H2O | - |
Trypanosoma congolense | 1-palmitoyl-sn-phosphatidylserine + palmitic acid | - |
? | |
| 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine + H2O | - |
Trypanosoma congolense | 1-palmitoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid | - |
? | |
| dimyristoyl phosphatidylcholine + H2O | - |
Trypanosoma congolense | 1-myristoyl-sn-glycerophosphocholine + myristic acid | - |
? | |
| dimyristoyl phosphatidylinositol + H2O | - |
Trypanosoma congolense | 1-myristoyl-sn-glycerophosphoinositol + myristic acid | - |
? | |
| dimyristoyl phosphatidylserinel + H2O | - |
Trypanosoma congolense | 1-myristoyl-sn-glycerophosphoserine + myristic acid | - |
? | |
| dioleolyl-sn- phosphatidylcholine + H2O | - |
Trypanosoma congolense | 1-oleolyl-sn-glycerophosphocholine + oleic acid | - |
? | |
| dioleolyl-sn- phosphatidylethanolamine + H2O | - |
Trypanosoma congolense | 1-oleolyl-sn-glycerophosphoethanolamine + oleic acid | - |
? | |
| dipalmitoyl- phosphatidylcholine + H2O | - |
Trypanosoma congolense | 1-palmitoyl-sn-glycerophosphocholine + palmitic acid | - |
? | |
| dipalmitoyl- phosphatidylethanolamine + H2O | - |
Trypanosoma congolense | 1-palmitoyl-sn-glycerophosphoethanolamine + palmitic acid | - |
? | |
| phospholipids + H2O | hemolytic activity of basic PLA2 | Trypanosoma congolense | ? | - |
? | |
| phospholipids + H2O | influence on platelet aggregation | Trypanosoma congolense | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 16500 + 1 * 18000, SDS-PAGE | Trypanosoma congolense |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Trypanosoma congolense |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme retains 70% of activity after 10 min incubation at 50°C, inactivation above 50°C | Trypanosoma congolense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 6.8 | - |
Trypanosoma congolense |
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