Cloned (Comment) | Organism |
---|---|
into pET21a vector, transformation of Escherichia coli strain BL21(DE3) | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
secretory granule | - |
Homo sapiens | 30141 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
heparin-sepharose affinity chromatography, SDS-PAGE after digestion with thrombin, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipids + H2O | Homo sapiens | eicosanoids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-4°C, more than 2 weeks without precipitation | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphatidic acid + H2O | group V sPLA2 | Homo sapiens | ? | - |
? | |
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphocholine + H2O | group V sPLA2 | Homo sapiens | 1-hexadecanoyl-glycero-3-phosphocholine + 1-pyrenedecanoate | - |
? | |
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoethanolamine + H2O | group V sPLA2 | Homo sapiens | 1-hexadecanoyl-glycero-3-phosphoethanolamine + 1-pyrenedecanoate | - |
? | |
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoglycerol + H2O | group V sPLA2 | Homo sapiens | 1-hexadecanoyl-glycero-3-phosphoglycerol + 1-pyrenedecanoate | - |
? | |
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoserine + H2O | group V sPLA2 | Homo sapiens | 1-hexadecanoyl-glycero-3-phosphoserine + 1-pyrenedecanoate | - |
? | |
phospholipids + H2O | eicosanoids | Homo sapiens | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
group V sPLA2 shows a half-life for inactivation of 10 min., group IIa sPLA2 retains 75% of activity after 1 h | Homo sapiens |