Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.54 | - |
methyl-3-(4-hydroxyphenyl)-propionate | pH 7.0 | Acinetobacter calcoaceticus | |
6.88 | - |
methyl cetraxate hydrochloride | pH 7.0 | Acinetobacter calcoaceticus | |
25.9 | - |
methyl-DL-beta-acetylthioisobutyrate | pH 7.0 | Acinetobacter calcoaceticus | |
54.1 | - |
dimethyl-(R)-methylsuccinate | pH 7.0 | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrocoumarin + H2O | Acinetobacter calcoaceticus | - |
melilotate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Acinetobacter calcoaceticus F45 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus | 3-(2-hydroxyphenyl)propionic acid | trivial name melilotate | ? | |
3,4-dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus F45 | 3-(2-hydroxyphenyl)propionic acid | trivial name melilotate | ? | |
dihydrocoumarin + H2O | - |
Acinetobacter calcoaceticus | melilotate | - |
? | |
dimethyl-(R)-methylsuccinate + H2O | 20.7% of activity with methyl-DL-beta-acetylthioisobutyrate | Acinetobacter calcoaceticus | (R)-methylsuccinate + methanol | - |
? | |
dimethyl-(R)-methylsuccinate + H2O | 20.7% of activity with methyl-DL-beta-acetylthioisobutyrate | Acinetobacter calcoaceticus F45 | (R)-methylsuccinate + methanol | - |
? | |
DL-methyl-2-chloropropionate + H2O | 1.6% of activity with methyl-DL-beta-acetylthioisobutyrate | Acinetobacter calcoaceticus | 2-chloropropionate + methanol | - |
? | |
DL-methyl-2-chloropropionate + H2O | 1.6% of activity with methyl-DL-beta-acetylthioisobutyrate | Acinetobacter calcoaceticus F45 | 2-chloropropionate + methanol | - |
? | |
methyl cetraxate hydrochloride + H2O | - |
Acinetobacter calcoaceticus | cetraxate hydrochloride + methanol | - |
? | |
methyl-(R)-3-bromo-2-methylpropionate + H2O | 3.5% of activity with methyl-DL-beta-acetylthioisobutyrate | Acinetobacter calcoaceticus | (R)-3-bromomethylpropionate + methanol | - |
? | |
methyl-(R)-3-bromo-2-methylpropionate + H2O | 3.5% of activity with methyl-DL-beta-acetylthioisobutyrate | Acinetobacter calcoaceticus F45 | (R)-3-bromomethylpropionate + methanol | - |
? | |
methyl-3(4-hydroxyphenyl)-propionate + H2O | 18.5% of activity with methyl-DL-beta-acetylthioisobutyrate | Acinetobacter calcoaceticus | 3-(4-hydroxyphenyl)-propionate + methanol | - |
? | |
methyl-DL-beta-acetylthioisobutyrate + H2O | stereospecific hydrolysis | Acinetobacter calcoaceticus | D-beta-acetylthioisobutyrate + methanol | - |
? | |
methyl-DL-beta-acetylthioisobutyrate + H2O | stereospecific hydrolysis | Acinetobacter calcoaceticus F45 | D-beta-acetylthioisobutyrate + methanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3,4-dihydrocoumarin hydrolase | - |
Acinetobacter calcoaceticus |