Literature summary for 3.1.1.31 extracted from

Hoelscher, C.; Meyer, T.; Von Schaewen, A.
Dual-targeting of Arabidopsis 6-phosphogluconolactonase 3 (PGL3) to chloroplasts and peroxisomes involves interaction with Trx m2 in the cytosol (2014), Mol. Plant, 7, 252-255.

Cloned(Commentary)

Cloned (Comment)	Organism
gene PGL3, presence of the C-terminal SKL motif interfered with chloroplast import of a PGL3_N-full-CFP fusion and with that of a PGL3_long medial-CFP fusion (with free N- and C-terminal ends) exclusively labeling peroxisomes, co-expression of thioredoxin Trxm2 with C-terminally truncated enzyme PGL3_N-short and _N-long versions accumulate in the cytosol of tobacco protoplasts and co-localization with Trxm2 in the cytosol and in the chloroplast stroma, recombinant expression of N-terminally CFP-tagged, green-fluorescent enzyme in Nicotiana benthamiana leaves	Arabidopsis thaliana

Cloned (Comment)

Organism

gene PGL3, presence of the C-terminal SKL motif interfered with chloroplast import of a PGL3_N-full-CFP fusion and with that of a PGL3_long medial-CFP fusion (with free N- and C-terminal ends) exclusively labeling peroxisomes, co-expression of thioredoxin Trxm2 with C-terminally truncated enzyme PGL3_N-short and _N-long versions accumulate in the cytosol of tobacco protoplasts and co-localization with Trxm2 in the cytosol and in the chloroplast stroma, recombinant expression of N-terminally CFP-tagged, green-fluorescent enzyme in Nicotiana benthamiana leaves

Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast stroma	co-localization with thioredoxin m-type, Trx_m2	Arabidopsis thaliana	9570	-
cytosol	enzyme precursor	Arabidopsis thaliana	5829	-
additional information	PGL3 contains an N-terminal extension and a C-terminal peroxisome-targeting motif PTS1, subcellular localization of enzyme stages, overview. Besides redox-related and metabolic signals that influence dual-targeting of enzyme PGL3 in leaves, developmental and/or physiological state(s) play another role for subcellular enzyme localization in different plant parts, the metabolic state affects PGL3 targeting by absence/reduced stability of thioredoxin Trxm2	Arabidopsis thaliana	-	-
peroxisome	co-localization with thioredoxin m-type, Trx_m2	Arabidopsis thaliana	5777	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	gene PGl3	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-

Synonyms

Synonyms	Comment	Organism
6-phosphogluconolactonase 3	-	Arabidopsis thaliana
PGL3	-	Arabidopsis thaliana