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Literature summary for 3.1.1.31 extracted from

  • Jortzik, E.; Mailu, B.M.; Preuss, J.; Fischer, M.; Bode, L.; Rahlfs, S.; Becker, K.
    Glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase: a unique bifunctional enzyme from Plasmodium falciparum (2011), Biochem. J., 436, 641-650.
    View publication on PubMed
Search for more literature for 3.1.1.31

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the gene encoding PfGluPho from chromosome 14 of the 3D7 gametocyte cDNA library, heterologous overexpression of PfGluPho using vector pQE30 in Escherichia coli strain M15 Plasmodium falciparum
gene h6PGL, overexpression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 using vector pRAREII Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
glutathione PfGluPho is inhibited by S-glutathionylation Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information detailed kinetic analysis of the bifunctional glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase indicates that PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-glucono-1,5-lactone + H2O Plasmodium falciparum
-
 6-phospho-D-gluconate
-
 ?
6-phospho-D-glucono-1,5-lactone + H2O Homo sapiens
-
 6-phospho-D-gluconate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O95336
-
-
Plasmodium falciparum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate PfGluPho functions on the basis of a rapid equilibrium random Bi Bi mechanism, where the binding of the second substrate depends on the first substrate Plasmodium falciparum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46
-
 recombinant PfGluPho, substrate is 6-phosphoglucono-gamma-lactone, pH 7.0, 25°C Plasmodium falciparum
60
-
 recombinant PfGluPho, substrate is 6-phosphoglucono-delta-lactone, pH 7.0, 25°C Plasmodium falciparum
1065
-
 recombinant h6PGL, substrate is 6-phospho-D-glucono-1,5-lactone, pH 7.0, 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-glucono-1,4-lactone + H2O the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring Plasmodium falciparum 6-phospho-D-gluconate
-
 ?
6-phospho-D-glucono-1,4-lactone + H2O the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring Homo sapiens 6-phospho-D-gluconate
-
 ?
6-phospho-D-glucono-1,5-lactone + H2O
-
 Plasmodium falciparum 6-phospho-D-gluconate
-
 ?
6-phospho-D-glucono-1,5-lactone + H2O
-
 Homo sapiens 6-phospho-D-gluconate
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphogluconolactonase
-
 Homo sapiens
6PGL
-
 Homo sapiens
glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase
-
 Plasmodium falciparum
GluPho
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Plasmodium falciparum
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Plasmodium falciparum
7
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism in Plasmodium falciparum, glucose-6-phosphate dehydrogenase is combined with the second enzyme of the pentose phosphate pathway to create a unique bifunctional enzyme named GluPho, glucose-6-phosphate dehydrogenase/6-phosphogluconolactonase. The G6PD activity is not largely influenced by the 6PGL part Plasmodium falciparum