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Literature summary for 3.1.1.3 extracted from

  • Belhaj, I.; Amara, S.; Parsiegla, G.; Sutto-Ortiz, P.; Sahaka, M.; Belghith, H.; Rousset, A.; Lafont, D.; Carriere, F.
    Galactolipase activity of Talaromyces thermophilus lipase on galactolipid micelles, monomolecular films and UV-absorbing surface-coated substrate (2018), Biochim. Biophys. Acta, 1863, 1006-1015 .
    View publication on PubMed
Search for more literature for 3.1.1.3

Organism

Organism UniProt Comment Textmining
Thermomyces dupontii F6LQK7
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Thermomyces dupontii Stolk F6LQK7
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-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain Stolk to homogeneity Thermomyces dupontii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4250
-
 substrate phosphatidylcholine, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
7300
-
 substrate tributyrin, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
9808
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 substrate olive oil, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii
24110
-
 substrate trioctanoin, pH 8.0, 37°C, purified native enzyme Thermomyces dupontii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Talaromyces thermophilus lipase (TTL) also hydrolyzes monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates (EC 3.1.1.26) presented in various forms to the enzyme. The enzyme has galactolipase activity on galactolipid micelles, monomolecular films, and UV-absorbing surface-coated substrate, overview. TTL possesses broad substrate specificity and is active on triglycerides with various acyl chain lengths, phospholipids, and galactolipids Thermomyces dupontii ?
-
 ?
additional information Talaromyces thermophilus lipase (TTL) also hydrolyzes monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates (EC 3.1.1.26) presented in various forms to the enzyme. The enzyme has galactolipase activity on galactolipid micelles, monomolecular films, and UV-absorbing surface-coated substrate, overview. TTL possesses broad substrate specificity and is active on triglycerides with various acyl chain lengths, phospholipids, and galactolipids Thermomyces dupontii Stolk ?
-
 ?
olive oil + H2O
-
 Thermomyces dupontii ?
-
 ?
olive oil + H2O
-
 Thermomyces dupontii Stolk ?
-
 ?
phosphatidylcholine + H2O from hen egg Thermomyces dupontii ?
-
 ?
phosphatidylcholine + H2O from hen egg Thermomyces dupontii Stolk ?
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 ?
tributyrin + H2O
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 Thermomyces dupontii dibutyrin + butyrate
-
 ?
tributyrin + H2O
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 Thermomyces dupontii Stolk dibutyrin + butyrate
-
 ?
trioctanoin + H2O
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 Thermomyces dupontii dioctanoin + octanoate
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 ?
trioctanoin + H2O
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 Thermomyces dupontii Stolk dioctanoin + octanoate
-
 ?

Synonyms

Synonyms Comment Organism
Talaromyces thermophilus lipase
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 Thermomyces dupontii
TTL
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 Thermomyces dupontii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Thermomyces dupontii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermomyces dupontii

General Information

General Information Comment Organism
additional information three-dimensional structure molecular modelling and dynamic light scattering measurements, sequence comparisons, overview Thermomyces dupontii