Literature summary for 3.1.1.3 extracted from

Crooks, G.E.; Rees, G.D.; Robinson, B.H.; Svensson, M.; Stephenson, G.R.
Comparison of hydrolysis and esterification behavior of Humicola lanuginosa and Rhizomucor miehei lipases in AOT-stabilized water-in-oil microemulsions II. Effect of temperature on reaction kinetics and general considerations of stability and productivit (1995), Biotechnol. Bioeng., 48, 190-196 .

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Application

Application	Comment	Organism
synthesis	solubilization in sodium bis-(2-ethylhexyl)sulfosuccinate-stabilized water-in-oil microemulsions in n-heptane and analysis of hydrolysis and condensation activity. Condensation activity is essentially independent of temperature over the range 5 to 37°C. The stability over a 30-day period is very good at all pH levels (6.1, 7.2, 9.3) and R values studied (5, 7.5, 10, 20), except when high pHs and low R values are combined	Thermomyces lanuginosus
synthesis	solubilization in sodium bis-(2-ethylhexyl)sulfosuccinate-stabilized water-in-oil microemulsions in n-heptane and analysis of hydrolysis and condensation activity. Esterification activity shows only a slight dependence on temperature over the studied range and an apparent activation energy of 20 kJ/mol for octyl decanoate synthesis. The enzyme shows good stability over a 30-day period in R = 7.5 and R = 10 microemulsions, pH 6.1	Rhizomucor miehei

Organism

Organism	UniProt	Comment	Textmining
Rhizomucor miehei	P19515	-	-
Thermomyces lanuginosus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Thermomyces lanuginosus	-
commercial preparation	-	Rhizomucor miehei	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl butanoate + H2O	-	Thermomyces lanuginosus	4-nitrophenol + butanoate	-	?
4-nitrophenyl butanoate + H2O	-	Rhizomucor miehei	4-nitrophenol + butanoate	-	?
octanol + decanoate	-	Rhizomucor miehei	octyl decanoate + H2O	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Rhizomucor miehei

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	poor stability at alkaline pH	Rhizomucor miehei

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Release 2023.1 (January 2023)