Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 5 mM, 51% residual activity | Saccharolobus solfataricus | |
diethyl dicarbonate | 0.5 mM, 15% residual activity | Saccharolobus solfataricus | |
eserine | 5 mM, 8% residual activity | Saccharolobus solfataricus | |
phenylmethylsulfonyl fluoride | 0.5 mM, 46% residual activity | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0241 | - |
4-nitrophenyl palmitate | pH 6.0, 70°C | Saccharolobus solfataricus | |
0.0466 | - |
4-nitrophenyl caprate | pH 6.0, 70°C | Saccharolobus solfataricus | |
0.129 | - |
4-nitrophenyl butanoate | pH 6.0, 70°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus DSM 1616 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2108 | - |
pH 6.0, 70°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butanoate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Saccharolobus solfataricus DSM 1616 | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Saccharolobus solfataricus DSM 1616 | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + palmitate | best substrate | ? | |
4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus DSM 1616 | 4-nitrophenol + palmitate | best substrate | ? | |
additional information | enzyme shows broad substrate specificity, exhibiting carboxylesterase activity towards short-chain acyl esters and lipase activity toward long-chain acyl esters including triacylglycerols regardless of saturated and unsaturated fatty acids | Saccharolobus solfataricus | ? | - |
? | |
additional information | enzyme shows broad substrate specificity, exhibiting carboxylesterase activity towards short-chain acyl esters and lipase activity toward long-chain acyl esters including triacylglycerols regardless of saturated and unsaturated fatty acids | Saccharolobus solfataricus DSM 1616 | ? | - |
? | |
triolein + 3 H2O | - |
Saccharolobus solfataricus | glycerol + 3 oleate | enzyme can hydrolyze all positions of the ester bonds in triolein | ? | |
triolein + 3 H2O | - |
Saccharolobus solfataricus DSM 1616 | glycerol + 3 oleate | enzyme can hydrolyze all positions of the ester bonds in triolein | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
substrate 4-nitrophenyl palmitate | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to 120 h | Saccharolobus solfataricus |
80 | - |
120 h, 50% residual activity | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1135 | - |
4-nitrophenyl butanoate | pH 6.0, 70°C | Saccharolobus solfataricus | |
1576 | - |
4-nitrophenyl caprate | pH 6.0, 70°C | Saccharolobus solfataricus | |
2252 | - |
4-nitrophenyl palmitate | pH 6.0, 70°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8800 | - |
4-nitrophenyl butanoate | pH 6.0, 70°C | Saccharolobus solfataricus | |
33800 | - |
4-nitrophenyl caprate | pH 6.0, 70°C | Saccharolobus solfataricus | |
93400 | - |
4-nitrophenyl palmitate | pH 6.0, 70°C | Saccharolobus solfataricus |