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Literature summary for 3.1.1.3 extracted from

  • Sharma, P.K.; Singh, K.; Singh, R.; Capalash, N.; Ali, A.; Mohammad, O.; Kaur, J.
    Characterization of a thermostable lipase showing loss of secondary structure at ambient temperature (2012), Mol. Biol. Rep., 39, 2795-2804.
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 49% residual activity at 1 mM uncultured bacterium
Cu2+ complete inhibition at 0.1 mM uncultured bacterium
diethyl dicarbonate complete inhibition at 0.1 mM uncultured bacterium
Hg2+ complete inhibition at 0.1 mM uncultured bacterium
Li+ 88% residual activity at 1 mM uncultured bacterium
Mg2+ 62% residual activity at 0.1 mM uncultured bacterium
Mn2+ 92% residual activity at 1 mM uncultured bacterium
additional information not inhibited by phenylmethylsulfonyl fluoride, eserine, and SDS uncultured bacterium
Triton X-100 the protein retains about 70% activity with Triton X-100 uncultured bacterium
Zn2+ 58% residual activity at 1 mM uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00073
-
 4-nitrophenyl laurate pH 8.0, 50°C uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 163% activity at 0.1 mM uncultured bacterium
Co2+ 124% activity at 0.1 mM uncultured bacterium
Li+ 112% activity at 0.1 mM uncultured bacterium
Zn2+ 162% activity at 0.1 mM uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, calculated from amino acid sequence uncultured bacterium

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone the enzyme displays 100% activity in presence of 30% (v/v) acetone uncultured bacterium
hexane the enzyme displays 100% activity in presence of 30% (v/v) n-hexane uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium B6VGJ3
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and phenyl Sepharose column chromatography uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
 enzyme from supernatant, using 4-nitrophenyl laurate as substrate, at pH 8.0, 50°C uncultured bacterium
2022
-
 enzyme after 61fold purification, using 4-nitrophenyl laurate as substrate, at pH 8.0, 50°C uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl decanoate + H2O about 25% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl laurate + H2O highest activity (100%) uncultured bacterium 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O about 50% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + myristate
-
 ?
4-nitrophenyl octanoate + H2O about 3% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl palmitate + H2O about 18% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl stearate + H2O about 6% activity compared to 4-nitrophenyl laurate uncultured bacterium 4-nitrophenol + stearate
-
 ?
additional information no activity with 4-nitrophenyl butyrate and 4-nitrophenyl propionate uncultured bacterium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, calculated from amino acid sequence uncultured bacterium

Synonyms

Synonyms Comment Organism
lipase
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme shows thermal stability up to 40°C without any loss of enzyme activity. Nearly 80% enzyme activity is retained at 50°C even after incubation for 75 min. However, above 50°C the enzyme displays thermal instability. At 55°C, enzyme activity almost reduces to 50, 44 and 32% after incubation for 45, 60 and 75 min, respectively. The half life of the enzyme is 5 min at 60°C uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
569
-
 4-nitrophenyl laurate pH 8.0, 50°C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 uncultured bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 the enzyme is completely stable at pH 8.0 and 9.0 when incubated at 22°C for 1 h, while nearly 50% enzyme activity is retained at pH 7.0, 10.0 and 11.0 uncultured bacterium