Cloned (Comment) | Organism |
---|---|
- |
Moesziomyces antarcticus |
Protein Variants | Comment | Organism |
---|---|---|
D134S | the mutant shows increased activity compared to the wild type enzyme | Moesziomyces antarcticus |
I189L | the mutant shows wild type activity | Moesziomyces antarcticus |
Q157A | the mutant shows increased activity compared to the wild type enzyme | Moesziomyces antarcticus |
Q157I | the mutant shows decreased activity compared to the wild type enzyme | Moesziomyces antarcticus |
Q157L | the mutant shows decreased activity compared to the wild type enzyme | Moesziomyces antarcticus |
Q157N | the mutant shows decreased activity compared to the wild type enzyme | Moesziomyces antarcticus |
Q157S | the mutant shows increased activity compared to the wild type enzyme | Moesziomyces antarcticus |
Q157T | the mutant shows increased activity compared to the wild type enzyme | Moesziomyces antarcticus |
T138A | the mutant shows decreased activity compared to the wild type enzyme | Moesziomyces antarcticus |
T138S | the mutant shows a 5fold increase in hydrolysis of isononanoic acid ethyl ester, but not toward sterically more demanding 2-ethyl hexanoic acid ethyl ester | Moesziomyces antarcticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, about 50000 Da, SDS-PAGE | Moesziomyces antarcticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moesziomyces antarcticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Moesziomyces antarcticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the wild type enzyme shows 741, 3.1 and 0.5 units/mg specific activity towards methyl caprylate, isononanoic acid ethyl ester and 2-ethyl hexanonic acid ethyl ester, respectively. Mutant enzyme T138S shows 427, 15.3 and 0.6 units/mg specific activity towards methyl caprylate, isononanoic acid ethyl ester and 2-ethyl hexanonic acid ethyl ester, respectively | Moesziomyces antarcticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-ethylhexanoic acid ethyl ester + H2O | - |
Moesziomyces antarcticus | (R)-2-ethylhexanoate + ethanol | - |
? | |
(R)-3,5,5-trimethylhexanoic acid ethyl ester + H2O | - |
Moesziomyces antarcticus | (R)-3,5,5-trimethylhexanoate + ethanol | - |
? | |
(S)-2-ethyl hexanoic acid ethyl ester + H2O | - |
Moesziomyces antarcticus | ? | - |
? | |
(S)-3,5,5-trimethylhexanoic acid ethyl ester + H2O | - |
Moesziomyces antarcticus | (S)-3,5,5-trimethylhexanoate + ethanol | - |
? | |
2-ethylhexanoic acid ethyl ester + H2O | lowest activity | Moesziomyces antarcticus | ? | - |
? | |
diethyl malate + H2O | - |
Moesziomyces antarcticus | ? | - |
? | |
diethyl tartrate + H2O | - |
Moesziomyces antarcticus | ? | - |
? | |
dimethyl succinate + H2O | high activity | Moesziomyces antarcticus | ? | - |
? | |
ethyl L-lactate + H2O | - |
Moesziomyces antarcticus | ethanol + L-lactate | - |
? | |
isononanoic acid ethyl ester + H2O | - |
Moesziomyces antarcticus | isononanoic acid + ethanol | - |
? | |
L-lysine ethyl ester + H2O | - |
Moesziomyces antarcticus | L-lysine + ethanol | - |
? | |
methyl caprylate + H2O | best substrate | Moesziomyces antarcticus | methanol + caprylate | - |
? | |
methyl laurate + H2O | - |
Moesziomyces antarcticus | methanol + laurate | - |
? | |
additional information | lipase B can only convert straight chain fatty acids of different length with a preference toward C5 and C12 | Moesziomyces antarcticus | ? | - |
? | |
triethyl citrate + H2O | - |
Moesziomyces antarcticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about 50000 Da, SDS-PAGE | Moesziomyces antarcticus |
Synonyms | Comment | Organism |
---|---|---|
lipase B | - |
Moesziomyces antarcticus |
Novozyme CALB L | commercial preparation | Moesziomyces antarcticus |