Application | Comment | Organism |
---|---|---|
biofuel production | Candida rugosa lipase immobilized on hydrous niobium oxide to be used in the biodiesel synthesis | Diutina rugosa |
General Stability | Organism |
---|---|
following immobilization by covalent attachment on hydrous niobium oxide, the enzyme exhibits improved storage stability and performs better at higher incubation temperatures. In addition, the enzyme retains most of its catalytic efficiency after successive operational cycles. For the esterification reaction of butanol with butyric acid (24 h, 37°C), a slow decrease in the esterification activity is verified (25%) after ten recycles (240 h), which corresponds to a half-life of 406 h | Diutina rugosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | - |
Diutina rugosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
4-nitrophenyl palmitate | free enzyme, pH 7.0, 37°C | Diutina rugosa | |
0.08923 | - |
4-nitrophenyl palmitate | immobilized enzyme, pH 7.0, 37°C | Diutina rugosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Diutina rugosa | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, niobium oxide-immobilized enzyme, 60 days, the activity decreases by 80% | Diutina rugosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Diutina rugosa | 4-nitrophenol + palmitate | - |
? | |
additional information | in the conversion of babassu oil into alkyl esters, butanol gives the highest conversion (79.35%). Babassu oil consists of 3.5% caprylic, 4.5% capric, 44.7% lauric, 17.5% myristic, 9.7% palmitic, 3.1% stearic, 15.2% oleic, and 1.8% linoleic acid | Diutina rugosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Diutina rugosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
free enzyme, there is a rapid decrease of activity at temperatures above 37°C (about 70% and 40% residual activity at 40°C and 60°C, respectively) | Diutina rugosa |
55 | - |
immobilized enzyme | Diutina rugosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
free enzyme at 55°C shows a half-life of 0.38 h, maintaining 20% of its original activity after 1 h, whereas the half-life of the immobilized lipase at this temperature is 0.82 h | Diutina rugosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
immobilized enzyme | Diutina rugosa |
8 | - |
free enzyme | Diutina rugosa |