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Literature summary for 3.1.1.3 extracted from

  • Miranda, M.; Urioste, D.; Andrade Souza, L.T.; Mendes, A.A.; de Castro, H.F.
    Assessment of the morphological, biochemical, and kinetic properties for Candida rugosa lipase immobilized on hydrous niobium oxide to be used in the biodiesel synthesis (2011), Enzyme Res., 2011, 216435.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biofuel production Candida rugosa lipase immobilized on hydrous niobium oxide to be used in the biodiesel synthesis Diutina rugosa

General Stability

General Stability Organism
following immobilization by covalent attachment on hydrous niobium oxide, the enzyme exhibits improved storage stability and performs better at higher incubation temperatures. In addition, the enzyme retains most of its catalytic efficiency after successive operational cycles. For the esterification reaction of butanol with butyric acid (24 h, 37°C), a slow decrease in the esterification activity is verified (25%) after ten recycles (240 h), which corresponds to a half-life of 406 h Diutina rugosa

Inhibitors

Inhibitors Comment Organism Structure
ethanol
-
Diutina rugosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
4-nitrophenyl palmitate free enzyme, pH 7.0, 37°C Diutina rugosa
0.08923
-
4-nitrophenyl palmitate immobilized enzyme, pH 7.0, 37°C Diutina rugosa

Organism

Organism UniProt Comment Textmining
Diutina rugosa
-
-
-

Storage Stability

Storage Stability Organism
4°C, niobium oxide-immobilized enzyme, 60 days, the activity decreases by 80% Diutina rugosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl palmitate + H2O
-
Diutina rugosa 4-nitrophenol + palmitate
-
?
additional information in the conversion of babassu oil into alkyl esters, butanol gives the highest conversion (79.35%). Babassu oil consists of 3.5% caprylic, 4.5% capric, 44.7% lauric, 17.5% myristic, 9.7% palmitic, 3.1% stearic, 15.2% oleic, and 1.8% linoleic acid Diutina rugosa ?
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Diutina rugosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
free enzyme, there is a rapid decrease of activity at temperatures above 37°C (about 70% and 40% residual activity at 40°C and 60°C, respectively) Diutina rugosa
55
-
immobilized enzyme Diutina rugosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
free enzyme at 55°C shows a half-life of 0.38 h, maintaining 20% of its original activity after 1 h, whereas the half-life of the immobilized lipase at this temperature is 0.82 h Diutina rugosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
immobilized enzyme Diutina rugosa
8
-
free enzyme Diutina rugosa