Any feedback?
Literature summary for 3.1.1.3 extracted from
- Cloning, expression and characterization of a new lipase from Yarrowia lipolytica (2011), Biotechnol. Lett., 33, 2445-2452.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris GS115 cells | Yarrowia lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibition at 1 mM | Yarrowia lipolytica |  |
| Cu2+ | about 25% inhibition at 1 mM | Yarrowia lipolytica | |
| dithiothreitol | inhibition at 1 mM | Yarrowia lipolytica | |
| EDTA | inhibition at 1 mM | Yarrowia lipolytica | |
| Fe2+ | inhibition at 1 mM | Yarrowia lipolytica | |
| Hg2+ | almost complete inhibition at 1 mM | Yarrowia lipolytica | |
| Mg2+ | inhibition at 1 mM | Yarrowia lipolytica | |
| Ni2+ | almost complete inhibition at 1 mM | Yarrowia lipolytica | |
| phenylmethylsulfonyl fluoride | complete inhibition at 1 mM | Yarrowia lipolytica | |
| Zn2+ | slight inhibition at 1 mM | Yarrowia lipolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 50% stimulation of activity at 1 mM | Yarrowia lipolytica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | - |
- |
- |
| Yarrowia lipolytica CLIB 122 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 triolein + H2O | 1,3-specificity for triolein | Yarrowia lipolytica | 3 oleic acid + 2-monoolein + 1,3-diolein | - |
? | |
| 4-nitrophenyl acetate + H2O | about 34% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | about 34% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica CLIB 122 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | about 42% activity compared to 4-nitrophenyl butyrate | Yarrowia lipolytica | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | about 42% activity compared to 4-nitrophenyl butyrate | Yarrowia lipolytica CLIB 122 | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caprylate + H2O | about 70% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl decanoate + H2O | 100% activity | Yarrowia lipolytica | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl decanoate + H2O | 100% activity | Yarrowia lipolytica CLIB 122 | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | about 40% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | about 40% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica CLIB 122 | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | about 65% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl myristate + H2O | about 10% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica | 4-nitrophenol + myristate | - |
? | |
| 4-nitrophenyl oleate + H2O | about 3% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica | 4-nitrophenol + oleate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Yarrowia lipolytica | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | about 8% activity compared to 4-nitrophenyl decanoate | Yarrowia lipolytica | 4-nitrophenol + palmitate | - |
? | |
| additional information | no phospholipid-hydrolyzing activity is detected with Lip9 | Yarrowia lipolytica | ? | - |
? | |
| additional information | no phospholipid-hydrolyzing activity is detected with Lip9 | Yarrowia lipolytica CLIB 122 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Lip9 | - |
Yarrowia lipolytica |
| lipase | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Yarrowia lipolytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | - |
Yarrowia lipolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | about 80% activity at 25°C, about 90% activity at 30°C, about 85% activity at 35°C, about 72% activity at 40°C, about 35% activity at 45°C | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Yarrowia lipolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Yarrowia lipolytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | about 55% activity at pH 5.0, about 72% activity at pH 6.0, about 90% activity at pH 8.0, about 68% activity at pH 9.0 | Yarrowia lipolytica |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
