Literature summary for 3.1.1.3 extracted from

Ebrahimpour, A.; Rahman, R.N.; Basri, M.; Salleh, A.B.
High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM (2011), Biores. Technol., 102, 6972-6981.

Activating Compound

Activating Compound	Comment	Organism
EDTA	about 120% activity at 1 mM, about 175% activity at 5 mM	Geobacillus sp.
phenylmethylsulfonyl fluoride	slight stimulation of activity at 1 mM	Geobacillus sp.
Triton X-100	1% (v/v) Triton X-100 causes an increase of 20% in lipase activity	Geobacillus sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia Top10 cells	Geobacillus sp.

General Stability

General Stability	Organism
the lipase shows the highest activity and the lowest thermostability in Tris-HCl buffer	Geobacillus sp.

Inhibitors

Inhibitors	Comment	Organism
1-butanol	complete inhibition at 1% (v/v)	Geobacillus sp.
1-propanol	about 99% inhibition at 1% (v/v)	Geobacillus sp.
2-propanol	about 95% inhibition at 1% (v/v)	Geobacillus sp.
acetone	about 98% inhibition at 1% (v/v)	Geobacillus sp.
acetonitrile	about 99% inhibition at 1% (v/v)	Geobacillus sp.
Ag+	about 85% inhibition at 1 mM	Geobacillus sp.
benzyl alcohol	complete inhibition at 1% (v/v)	Geobacillus sp.
beta-mercaptoethanol	about 75% inhibition at 1 mM, about 99% inhibition at 5 mM	Geobacillus sp.
Co2+	about 75% inhibition at 5 mM	Geobacillus sp.
Cu2+	about 46% inhibition at 5 mM	Geobacillus sp.
Fe2+	about 60% inhibition at 5 mM	Geobacillus sp.
iso-amyl alcohol	about 80% inhibition at 1% (v/v)	Geobacillus sp.
iso-butanol	about 95% inhibition at 1% (v/v)	Geobacillus sp.
K+	about 48% inhibition at 5 mM	Geobacillus sp.
Li+	about 10% inhibition at 5 mM	Geobacillus sp.
Mg2+	about 30% inhibition at 1 mM	Geobacillus sp.
Mn2+	about 75% inhibition at 1 mM	Geobacillus sp.
additional information	Span 20 does not affect the activity	Geobacillus sp.
Na+	about 75% inhibition at 5 mM	Geobacillus sp.
Ni2+	about 45% inhibition at 5 mM	Geobacillus sp.
phenylmethylsulfonyl fluoride	about 99% inhibition at 5 mM	Geobacillus sp.
Rb+	about 10% inhibition at 5 mM	Geobacillus sp.
SDS	about 90% inhibition at 1% (w/v) SDS	Geobacillus sp.
Sr2+	about 45% inhibition at 5 mM	Geobacillus sp.
Triton B-1956	about 55% inhibition at 1% (w/v) Triton B-1956	Geobacillus sp.
Tween 20	about 90% inhibition at 1% (w/v) Tween 20	Geobacillus sp.
Tween 40	about 40% inhibition at 1% (w/v) Tween 40	Geobacillus sp.
Tween 80	about 50% inhibition at 1% (w/v) Tween 80	Geobacillus sp.
Tween 85	about 85% inhibition at 1% (w/v) Tween 85	Geobacillus sp.
Zn2+	about 57% inhibition at 5 mM	Geobacillus sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	about 150% activity at 1-10 mM	Geobacillus sp.
additional information	not a metalloenzyme	Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	1 * 44000, SDS-PAGE	Geobacillus sp.

Organic Solvent Stability

Organic Solvent	Comment	Organism
1-decanol	the enzyme retains its activity of 86% in the presence of 1% (v/v) 1-decanol	Geobacillus sp.
1-dodecanol	149.6% activity at 1% (v/v)	Geobacillus sp.
1-octanol	137.3% activity at 1% (v/v)	Geobacillus sp.
1-undecanol	the enzyme retains its activity of 74% in the presence of 1% (v/v) 1-undecanol	Geobacillus sp.
2,3-butanediol	100.4% activity at 1% (v/v)	Geobacillus sp.
benzene	142.0% activity at 1% (v/v)	Geobacillus sp.
DMSO	the enzyme retains its activity of 62.5% in the presence of 1% (v/v) DMSO	Geobacillus sp.
Ethanol	116.4% activity at 1% (v/v)	Geobacillus sp.
ethylbenzene	the enzyme retains its activity of 77% in the presence of 1% (v/v) ethylbenzene	Geobacillus sp.
heptane	149.6% activity at 1% (v/v)	Geobacillus sp.
hexane	139.3% activity at 1% (v/v)	Geobacillus sp.
isooctane	128.6% activity at 1% (v/v)	Geobacillus sp.
Methanol	107.3% activity at 1% (v/v)	Geobacillus sp.
n-heptadecane	142.5% activity at 1% (v/v)	Geobacillus sp.
n-hexacedane	the enzyme retains its activity of 78% in the presence of 1% (v/v) n-hexadecane	Geobacillus sp.
n-pentadecane	128.5% activity at 1% (v/v)	Geobacillus sp.
n-tetradecane	115.8% activity at 1% (v/v)	Geobacillus sp.
o-xylene	136.1% activity at 1% (v/v)	Geobacillus sp.
toluene	164.9% activity at 1% (v/v)	Geobacillus sp.

Organism

Organism	UniProt	Comment	Textmining
Geobacillus sp.	-	-	-
Geobacillus sp. ARM	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ProBond nickel-chelating resin chromatography with 63.2% recovery and purification factor of 14.6	Geobacillus sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
155.1	-	crude enzyme, at pH 8.0, 65°C	Geobacillus sp.
2259	-	after 14.6fold purification, at pH 8.0, 65°C	Geobacillus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	the lipase hydrolyzes triacylglycerol to produce 1,2-diacylglycerol (with yield of 50.2%) and 2-monoacylglycerol (with yield of 14.0%) as main products and free fatty acids	Geobacillus sp.	?	-	?
additional information	the lipase hydrolyzes triacylglycerol to produce 1,2-diacylglycerol (with yield of 50.2%) and 2-monoacylglycerol (with yield of 14.0%) as main products and free fatty acids	Geobacillus sp. ARM	?	-	?
triolein + H2O	cleavage occurs only at the Sn-1 and Sn-3 positions	Geobacillus sp.	?	-	?
triolein + H2O	cleavage occurs only at the Sn-1 and Sn-3 positions	Geobacillus sp. ARM	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 44000, SDS-PAGE	Geobacillus sp.
monomer	1 *43300, mature lipase, calculated from amino acid sequence	Geobacillus sp.

Synonyms

Synonyms	Comment	Organism
lipase	-	Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Geobacillus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Geobacillus sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	9	the enzyme retains 50% residual activity at 50°C after incubation at pH 6.0-7.0 for more than 150 min, pH 8.0 (PBS buffer) for 130 min, pH 8.0 (Tris-HCl buffer) for 90 min and pH 9.0 around 110 min	Geobacillus sp.