Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | about 120% activity at 1 mM, about 175% activity at 5 mM | Geobacillus sp. | |
phenylmethylsulfonyl fluoride | slight stimulation of activity at 1 mM | Geobacillus sp. | |
Triton X-100 | 1% (v/v) Triton X-100 causes an increase of 20% in lipase activity | Geobacillus sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia Top10 cells | Geobacillus sp. |
General Stability | Organism |
---|---|
the lipase shows the highest activity and the lowest thermostability in Tris-HCl buffer | Geobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanol | complete inhibition at 1% (v/v) | Geobacillus sp. | |
1-propanol | about 99% inhibition at 1% (v/v) | Geobacillus sp. | |
2-propanol | about 95% inhibition at 1% (v/v) | Geobacillus sp. | |
acetone | about 98% inhibition at 1% (v/v) | Geobacillus sp. | |
acetonitrile | about 99% inhibition at 1% (v/v) | Geobacillus sp. | |
Ag+ | about 85% inhibition at 1 mM | Geobacillus sp. | |
benzyl alcohol | complete inhibition at 1% (v/v) | Geobacillus sp. | |
beta-mercaptoethanol | about 75% inhibition at 1 mM, about 99% inhibition at 5 mM | Geobacillus sp. | |
Co2+ | about 75% inhibition at 5 mM | Geobacillus sp. | |
Cu2+ | about 46% inhibition at 5 mM | Geobacillus sp. | |
Fe2+ | about 60% inhibition at 5 mM | Geobacillus sp. | |
iso-amyl alcohol | about 80% inhibition at 1% (v/v) | Geobacillus sp. | |
iso-butanol | about 95% inhibition at 1% (v/v) | Geobacillus sp. | |
K+ | about 48% inhibition at 5 mM | Geobacillus sp. | |
Li+ | about 10% inhibition at 5 mM | Geobacillus sp. | |
Mg2+ | about 30% inhibition at 1 mM | Geobacillus sp. | |
Mn2+ | about 75% inhibition at 1 mM | Geobacillus sp. | |
additional information | Span 20 does not affect the activity | Geobacillus sp. | |
Na+ | about 75% inhibition at 5 mM | Geobacillus sp. | |
Ni2+ | about 45% inhibition at 5 mM | Geobacillus sp. | |
phenylmethylsulfonyl fluoride | about 99% inhibition at 5 mM | Geobacillus sp. | |
Rb+ | about 10% inhibition at 5 mM | Geobacillus sp. | |
SDS | about 90% inhibition at 1% (w/v) SDS | Geobacillus sp. | |
Sr2+ | about 45% inhibition at 5 mM | Geobacillus sp. | |
Triton B-1956 | about 55% inhibition at 1% (w/v) Triton B-1956 | Geobacillus sp. | |
Tween 20 | about 90% inhibition at 1% (w/v) Tween 20 | Geobacillus sp. | |
Tween 40 | about 40% inhibition at 1% (w/v) Tween 40 | Geobacillus sp. | |
Tween 80 | about 50% inhibition at 1% (w/v) Tween 80 | Geobacillus sp. | |
Tween 85 | about 85% inhibition at 1% (w/v) Tween 85 | Geobacillus sp. | |
Zn2+ | about 57% inhibition at 5 mM | Geobacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 150% activity at 1-10 mM | Geobacillus sp. | |
additional information | not a metalloenzyme | Geobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
1 * 44000, SDS-PAGE | Geobacillus sp. |
Organic Solvent | Comment | Organism |
---|---|---|
1-decanol | the enzyme retains its activity of 86% in the presence of 1% (v/v) 1-decanol | Geobacillus sp. |
1-dodecanol | 149.6% activity at 1% (v/v) | Geobacillus sp. |
1-octanol | 137.3% activity at 1% (v/v) | Geobacillus sp. |
1-undecanol | the enzyme retains its activity of 74% in the presence of 1% (v/v) 1-undecanol | Geobacillus sp. |
2,3-butanediol | 100.4% activity at 1% (v/v) | Geobacillus sp. |
benzene | 142.0% activity at 1% (v/v) | Geobacillus sp. |
DMSO | the enzyme retains its activity of 62.5% in the presence of 1% (v/v) DMSO | Geobacillus sp. |
Ethanol | 116.4% activity at 1% (v/v) | Geobacillus sp. |
ethylbenzene | the enzyme retains its activity of 77% in the presence of 1% (v/v) ethylbenzene | Geobacillus sp. |
heptane | 149.6% activity at 1% (v/v) | Geobacillus sp. |
hexane | 139.3% activity at 1% (v/v) | Geobacillus sp. |
isooctane | 128.6% activity at 1% (v/v) | Geobacillus sp. |
Methanol | 107.3% activity at 1% (v/v) | Geobacillus sp. |
n-heptadecane | 142.5% activity at 1% (v/v) | Geobacillus sp. |
n-hexacedane | the enzyme retains its activity of 78% in the presence of 1% (v/v) n-hexadecane | Geobacillus sp. |
n-pentadecane | 128.5% activity at 1% (v/v) | Geobacillus sp. |
n-tetradecane | 115.8% activity at 1% (v/v) | Geobacillus sp. |
o-xylene | 136.1% activity at 1% (v/v) | Geobacillus sp. |
toluene | 164.9% activity at 1% (v/v) | Geobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | - |
- |
- |
Geobacillus sp. ARM | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ProBond nickel-chelating resin chromatography with 63.2% recovery and purification factor of 14.6 | Geobacillus sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
155.1 | - |
crude enzyme, at pH 8.0, 65°C | Geobacillus sp. |
2259 | - |
after 14.6fold purification, at pH 8.0, 65°C | Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the lipase hydrolyzes triacylglycerol to produce 1,2-diacylglycerol (with yield of 50.2%) and 2-monoacylglycerol (with yield of 14.0%) as main products and free fatty acids | Geobacillus sp. | ? | - |
? | |
additional information | the lipase hydrolyzes triacylglycerol to produce 1,2-diacylglycerol (with yield of 50.2%) and 2-monoacylglycerol (with yield of 14.0%) as main products and free fatty acids | Geobacillus sp. ARM | ? | - |
? | |
triolein + H2O | cleavage occurs only at the Sn-1 and Sn-3 positions | Geobacillus sp. | ? | - |
? | |
triolein + H2O | cleavage occurs only at the Sn-1 and Sn-3 positions | Geobacillus sp. ARM | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Geobacillus sp. |
monomer | 1 *43300, mature lipase, calculated from amino acid sequence | Geobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Geobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the enzyme retains 50% residual activity at 50°C after incubation at pH 6.0-7.0 for more than 150 min, pH 8.0 (PBS buffer) for 130 min, pH 8.0 (Tris-HCl buffer) for 90 min and pH 9.0 around 110 min | Geobacillus sp. |