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Literature summary for 3.1.1.3 extracted from

  • Dheeman, D.S.; Henehan, G.T.; Frias, J.M.
    Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis (2011), Biores. Technol., 102, 3373-3379.
    View publication on PubMed
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid 124.4% activity at 1 mM Amycolatopsis mediterranei
beta-mercaptoethanol 136.4% activity at 1 mM Amycolatopsis mediterranei
Digitonin 142.5% activity at 1 mM Amycolatopsis mediterranei
dithiothreitol 147.6% activity at 1 mM Amycolatopsis mediterranei
sodium deoxycholate 241.3% activity at 1 mM Amycolatopsis mediterranei

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide complete inhibition at 5 mM Amycolatopsis mediterranei
capric acid 89.7% residual activity at 1 mM Amycolatopsis mediterranei
diethyldicarbonate complete inhibition at 5 mM Amycolatopsis mediterranei
Hg2+ Hg2+ shows the highest reduction in enzyme activity by 83.3% at 1 mM Amycolatopsis mediterranei
additional information not affected by EDTA, sodium citrate, SDS, myristic acid, palmitic acid, and urea (6.0 M). The enzyme is not inhibited by iodoacetate, citraconic anhydride, phenylglyoxal, and N-acetylimidazole Amycolatopsis mediterranei
N-bromosuccinimide 34.4% residual activity at 5 mM Amycolatopsis mediterranei
phenylmethylsulfonyl fluoride 11.6% residual activity at 5 mM Amycolatopsis mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.099
-
 4-nitrophenyl palmitate pH 8.0, 60°C Amycolatopsis mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Amycolatopsis mediterranei

Organism

Organism UniProt Comment Textmining
Amycolatopsis mediterranei
-
-
-
Amycolatopsis mediterranei DSM 43304
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q Sepharose column chromatography, and Toyopearl phenyl-650M column chromatography (398fold purification with 36% final recovery) Amycolatopsis mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.96
-
 enzyme from culture filtrate, using 4-nitrophenyl palmitate as substrate, at pH 8.0 and 60°C Amycolatopsis mediterranei
781.6
-
 enzyme after 398.79fold purification, using 4-nitrophenyl palmitate as substrate, at pH 8.0 and 60°C Amycolatopsis mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 triolein + H2O about 115% activity compared to olive oil Amycolatopsis mediterranei 6 oleic acid + 1,3-diolein + 1,2-diolein + 1-monoolein + 2-monoolein oleic acid is the major product ?
4-nitrophenyl acetate + H2O about 88% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O about 88% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caproate + H2O second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caproate + H2O second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei DSM 43304 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caprylate + H2O highest hydrolysis rate, about 90% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl laurate + H2O about 60% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O about 30% activity compared to 4-nitrophenyl palmitate Amycolatopsis mediterranei 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O the purified enzyme is most active toward 4-nitrophenyl palmitate (100% activity) Amycolatopsis mediterranei 4-nitrophenol + palmitate
-
 ?
isoamyl alcohol + acetic acid after 72 h of reaction a conversion yield of 34.4% and 16.2% of isoamyl acetate is obtained using cellite-immobilized and free enzyme, respectively Amycolatopsis mediterranei isoamyl acetate
-
 ?
additional information the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates Amycolatopsis mediterranei ?
-
 ?
additional information the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates Amycolatopsis mediterranei DSM 43304 ?
-
 ?
trilinolein + H2O about 105% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
trilinolenin + H2O about 100% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
trioctanoin + H2O about 59% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
trioctanoin + H2O about 59% activity compared to olive oil Amycolatopsis mediterranei DSM 43304 ?
-
 ?
tripalmitin + H2O about 45% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
tristearin + H2O about 38% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?
trivaccinin + H2O about 78% activity compared to olive oil Amycolatopsis mediterranei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Amycolatopsis mediterranei

Synonyms

Synonyms Comment Organism
lipase
-
 Amycolatopsis mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Amycolatopsis mediterranei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the purified enzyme is most stable at 50-60°C retaining 100% activity after 3 h Amycolatopsis mediterranei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1468
-
 4-nitrophenyl palmitate apparent value, in 50 mM Tris-HCl buffer, at pH 8.0, 60°C Amycolatopsis mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Amycolatopsis mediterranei

pH Range

pH Minimum pH Maximum Comment Organism
7 9 the activity is not much affected at pH 7.0 and 9.0 where it shows around 90% of relative activity Amycolatopsis mediterranei

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the purified enzyme is stable in the pH range 6.0-9.0 retaining more than 95% of relative activity after 24 h of incubation Amycolatopsis mediterranei