Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Gum arabic | 142% relative activity at 1% (w/v) | Serratia marcescens | |
Pg350me | 117% relative activity at 1% (w/v) | Serratia marcescens | |
polyvinyl alcohol | 118% relative activity at 1% (w/v) | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 98.3% inhibition at 50% (v/v) | Serratia marcescens | |
Ca2+ | 95.0% residual activity at 10 mM | Serratia marcescens | |
Co2+ | 46.0% residual activity at 10 mM | Serratia marcescens | |
Cu2+ | 37.0% residual activity at 10 mM | Serratia marcescens | |
EDTA | 16.0% residual activity at 10 mM | Serratia marcescens | |
ethanol | 98.8% inhibition at 50% (v/v) | Serratia marcescens | |
Fe2+ | 14.0% residual activity at 10 mM | Serratia marcescens | |
Fe3+ | 12% residual activity at 10 mM | Serratia marcescens | |
Isopropanol | 98.9% inhibition at 50% (v/v) | Serratia marcescens | |
K+ | 89.0% residual activity at 10 mM | Serratia marcescens | |
methanol | 99% inhibition at 50% (v/v) | Serratia marcescens | |
Mg2+ | 88.0% residual activity at 10 mM | Serratia marcescens | |
Mn2+ | 60.0% residual activity at 10 mM | Serratia marcescens | |
Na+ | 90.0% residual activity at 10 mM | Serratia marcescens | |
Ni2+ | 40.0% residual activity at 10 mM | Serratia marcescens | |
Pg250de | 78% residual activity at 1% (v/v) | Serratia marcescens | |
Pg400de | 96% residual activity at 1% (v/v) | Serratia marcescens | |
Triton X-100 | inhibits the activity seriously | Serratia marcescens | |
Triton X-45 | inhibits the activity seriously | Serratia marcescens | |
Tween 80 | 72% residual activity at 1% (v/v) | Serratia marcescens | |
Zn2+ | 16.2% residual activity at 10 mM | Serratia marcescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by Ca2+ | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Serratia marcescens |
140000 | - |
native lipase, gel filtration | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Serratia marcescens ECU1010 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Toyopearl 650 M column chromatography, Sephadex G150 gel filtration, and phenyl-Toyopearl 650 M column chromatography | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90 | - |
crude enzyme, using 4-nitrophenyl butyrate as substrate, in 100 mM potassium phosphate buffer (pH 7.0), at 30°C | Serratia marcescens |
497 | - |
after 5.5fold purification, using 4-nitrophenyl butyrate as substrate, in 100 mM potassium phosphate buffer (pH 7.0), at 30°C | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one acetate + H2O | 50% conversion | Serratia marcescens | ? | - |
? | |
4-nitrophenyl acetate + H2O | - |
Serratia marcescens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Serratia marcescens ECU1010 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Serratia marcescens | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Serratia marcescens ECU1010 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Serratia marcescens | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Serratia marcescens | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Serratia marcescens ECU1010 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Serratia marcescens | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | the enzyme shows maximum activity on 4-nitrophenyl myristate | Serratia marcescens | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Serratia marcescens | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Serratia marcescens | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Serratia marcescens ECU1010 | 4-nitrophenol + propionate | - |
? | |
4-phenyl-[1,3]-dioxolan-2-one + H2O | 9.6% conversion | Serratia marcescens | ? | - |
? | |
glycidyl butyrate + H2O | 49% conversion | Serratia marcescens | ? | - |
? | |
methyl acetate + H2O | 25% conversion | Serratia marcescens | ? | - |
? | |
methyl mandelate + H2O | 33% conversion | Serratia marcescens | ? | - |
? | |
additional information | does not hydolyze propylene carbonate | Serratia marcescens | ? | - |
? | |
additional information | does not hydolyze propylene carbonate | Serratia marcescens ECU1010 | ? | - |
? | |
naproxen methyl ester + H2O | 41% conversion | Serratia marcescens | ? | - |
? | |
trans-3-(4'-methoxyphenyl) glycidic acid methyl ester + H2O | 60% conversion | Serratia marcescens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 65000, native lipase, gel filtration | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Serratia marcescens |
triacylglycerol hydrolase | - |
Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
retains 71% activity after incubation at 50°C for 1 h | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Serratia marcescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Serratia marcescens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Serratia marcescens | isoelectric focusing | - |
4.2 |