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Literature summary for 3.1.1.3 extracted from

  • Gatti-Lafranconi, P.; Caldarazzo, S.M.; Villa, A.; Alberghina, L.; Lotti, M.
    Unscrambling thermal stability and temperature adaptation in evolved variants of a cold-active lipase (2008), FEBS Lett., 582, 2313-2318.
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Pseudomonas fragi

Protein Variants

Protein Variants Comment Organism
D2Y/H30N mutant shows elevated thermal stability with half-lives of 125 min at 37°C and 44 min at 42°C Pseudomonas fragi
R127G/R152H mutant shows elevated thermal stability with half-lives of 98 min at 37°C and 38 min at 42°C Pseudomonas fragi
R127G/R152H/D2Y/H30N mutant shows elevated thermal stability with half-lives of more than 4 h at 37°C and more than 100 min at 42°C Pseudomonas fragi
R127G/R152H/K205Q/S271T mutant shows elevated thermal stability with half-lives of 154 min at 37°C and 47 min at 42°C Pseudomonas fragi
R127G/R152H/R70S/V121I/T131K/K205Q/S271T mutant shows elevated thermal stability with half-lives of 132 min at 37°C and 52 min at 42°C Pseudomonas fragi

Organism

Organism UniProt Comment Textmining
Pseudomonas fragi
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyceryl trioctanoate + H2O
-
 Pseudomonas fragi glycerol + octanoate
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 ?

Synonyms

Synonyms Comment Organism
lipase
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 Pseudomonas fragi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10
-
-
 Pseudomonas fragi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 42 the wild type enzyme displays high activity at 10°C, but it is easily inactivated with half-lives of 4 h at 29°C, 83 min at 37°C, and 19 min at 42°C Pseudomonas fragi