Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Karra-Chaabouni, M.; Bouaziz, I.; Boufi, S.; Botelho do Rego, A.M.; Gargouri, Y.
    Physical immobilization of Rhizopus oryzae lipase onto cellulose substrate: activity and stability studies (2008), Colloids Surf. B Biointerfaces, 66, 168-177.
    View publication on PubMed

General Stability

General Stability Organism
immobilized lipase carried on Cell-450 oxidized cellulose fibers shows an activity yield of 15% Rhizopus arrhizus

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by ammonium sulfate precipitation Rhizopus arrhizus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oleic acid + n-butanol
-
Rhizopus arrhizus ?
-
?
olive oil + H2O
-
Rhizopus arrhizus ?
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Rhizopus arrhizus
triacylglycerol ester hydrolase
-
Rhizopus arrhizus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 the activity of the free lipase is maintained roughly constant until temperature 37°C, above which a continuous decrease in the activity occurs, the activity is preserved till 45°C for the enzyme immobilized on Cell-450 oxidized cellulose fibers Rhizopus arrhizus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
around pH 8.3 Rhizopus arrhizus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5
-
Rhizopus arrhizus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.3 activity of the free lipase drops rapidly above pH 8.3, immobilized lipase is stable within the pH range 3-8, the free lipase is stable only at pH 6 Rhizopus arrhizus