Literature summary for 3.1.1.3 extracted from

Joseph, B.; Ramteke, P.W.; Thomas, G.
Cold active microbial lipases: some hot issues and recent developments (2008), Biotechnol. Adv., 26, 457-470.

Application

Application	Comment	Organism
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Aspergillus nidulans
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Lysinibacillus sphaericus
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Pseudomonas sp.
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Serratia marcescens
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Acinetobacter sp.
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Aeromonas sp.
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Psychrobacter sp.
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Pseudoalteromonas sp.
biofuel production	conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel	Microbacterium phyllosphaerae
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Aspergillus nidulans
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Lysinibacillus sphaericus
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Pseudomonas sp.
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Serratia marcescens
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Acinetobacter sp.
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Aeromonas sp.
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Psychrobacter sp.
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Pseudoalteromonas sp.
detergent	detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics	Microbacterium phyllosphaerae
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Aspergillus nidulans
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Lysinibacillus sphaericus
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Pseudomonas sp.
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Serratia marcescens
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Acinetobacter sp.
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Aeromonas sp.
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Psychrobacter sp.
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Pseudoalteromonas sp.
environmental protection	degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids	Microbacterium phyllosphaerae
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Aspergillus nidulans
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Lysinibacillus sphaericus
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Pseudomonas sp.
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Serratia marcescens
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Acinetobacter sp.
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Aeromonas sp.
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Psychrobacter sp.
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Pseudoalteromonas sp.
food industry	protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats	Microbacterium phyllosphaerae
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Aspergillus nidulans
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Lysinibacillus sphaericus
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Pseudomonas sp.
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Serratia marcescens
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Acinetobacter sp.
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Aeromonas sp.
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Psychrobacter sp.
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Pseudoalteromonas sp.
synthesis	synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide	Microbacterium phyllosphaerae

Cloned(Commentary)

Cloned (Comment)	Organism
-	Psychrobacter sp.
expressed in Escherichia coli	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism
Cu2+	strong inhibition	Pseudomonas sp.
Fe3+	strong inhibition	Pseudomonas sp.
Hg2+	strong inhibition	Pseudomonas sp.
Zn2+	strong inhibition	Pseudomonas sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	enhances activity	Psychrobacter sp.
Mg2+	enhances activity	Psychrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	-	Aspergillus nidulans
33700	-	-	Pseudomonas sp.
40000	-	SDS-PAGE	Lysinibacillus sphaericus
42000	-	-	Microbacterium phyllosphaerae
49900	-	-	Pseudomonas sp.
50000	-	SDS-PAGE	Aeromonas sp.
52000	-	-	Serratia marcescens
85000	-	-	Psychrobacter sp.
85000	-	-	Pseudoalteromonas sp.

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter sp.	-	strain no. 6	-
Aeromonas sp.	-	-	-
Aeromonas sp. LPB 4	-	-	-
Aspergillus nidulans	-	-	-
Aspergillus nidulans WG312	-	-	-
Lysinibacillus sphaericus	-	-	-
Lysinibacillus sphaericus MTCC 7526	-	-	-
Microbacterium phyllosphaerae	-	-	-
Microbacterium phyllosphaerae MTCC 7530	-	-	-
Pseudoalteromonas sp.	-	-	-
Pseudoalteromonas sp. wp27	-	-	-
Pseudomonas sp.	-	-	-
Pseudomonas sp. B11-1	-	-	-
Pseudomonas sp. KB700A	-	-	-
Psychrobacter sp.	-	-	-
Psychrobacter sp. 7195	-	-	-
Psychrobacter sp. wp37	-	-	-
Serratia marcescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
CM-cellulose column chromatography, DEAE cellulose column chromatography, and Sephadex G-150 gel filtration	Serratia marcescens
DEAE cellulofine chromatography	Pseudomonas sp.
DEAE cellulose column chromatography	Lysinibacillus sphaericus
DEAE cellulose column chromatography	Pseudomonas sp.
DEAE cellulose column chromatography	Microbacterium phyllosphaerae
DEAE Sepharose CL-4B column chromatography and Sephadex G-75 gel filtration	Psychrobacter sp.
phenyl-Sepharose column chromatography and linolenic acid-agarose chromatography	Aspergillus nidulans
QAE-Sephadex gel filtration	Aeromonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	broad specificity towards the acyl group (C8-C16) of ethyl esters	Acinetobacter sp.	?	-	?
additional information	medium chain acyl group p-nitrophenyl esters are good substrate, increased activity with detergents	Aeromonas sp.	?	-	?
additional information	preference toward esters of short- and middle-chain fatty acids	Aspergillus nidulans	?	-	?
additional information	preference toward esters of short- and middle-chain fatty acids	Aspergillus nidulans WG312	?	-	?
additional information	medium chain acyl group p-nitrophenyl esters are good substrate, increased activity with detergents	Aeromonas sp. LPB 4	?	-	?
p-nitrophenyl caprate + H2O	highest activity with p-nitrophenyl caprate	Pseudomonas sp.	p-nitrophenol + caprate	-	?
p-nitrophenyl caprate + H2O	highest activity with p-nitrophenyl caprate	Pseudomonas sp. KB700A	p-nitrophenol + caprate	-	?
p-nitrophenyl caprate + H2O	highest activity with p-nitrophenyl caprate	Pseudomonas sp. B11-1	p-nitrophenol + caprate	-	?

Synonyms

Synonyms	Comment	Organism
triacylglycerol acyl hydrolase	-	Aspergillus nidulans
triacylglycerol acyl hydrolase	-	Lysinibacillus sphaericus
triacylglycerol acyl hydrolase	-	Pseudomonas sp.
triacylglycerol acyl hydrolase	-	Serratia marcescens
triacylglycerol acyl hydrolase	-	Acinetobacter sp.
triacylglycerol acyl hydrolase	-	Aeromonas sp.
triacylglycerol acyl hydrolase	-	Psychrobacter sp.
triacylglycerol acyl hydrolase	-	Pseudoalteromonas sp.
triacylglycerol acyl hydrolase	-	Microbacterium phyllosphaerae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
15	-	-	Lysinibacillus sphaericus
20	-	-	Acinetobacter sp.
20	-	-	Microbacterium phyllosphaerae
20	30	-	Psychrobacter sp.
20	30	-	Pseudoalteromonas sp.
30	-	-	Psychrobacter sp.
35	-	-	Pseudomonas sp.
35	-	-	Aeromonas sp.
37	-	-	Serratia marcescens
40	-	-	Aspergillus nidulans
45	-	-	Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	enzyme is 60% active at 4°C	Pseudomonas sp.
4	-	enzyme is 60% active at 4°C	Psychrobacter sp.
4	-	enzyme is 60% active at 4°C	Pseudoalteromonas sp.
5	35	-	Pseudomonas sp.
5	65	90% activity at 5°C, remains stable at 65°C	Serratia marcescens
30	-	stable at 30°C	Lysinibacillus sphaericus
35	-	stable at 35°C	Microbacterium phyllosphaerae
50	-	stable at 50°C	Aeromonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Aspergillus nidulans
7	8	-	Psychrobacter sp.
7	8	-	Pseudoalteromonas sp.
8	-	-	Lysinibacillus sphaericus
8	-	-	Pseudomonas sp.
8	-	-	Serratia marcescens
8	-	-	Microbacterium phyllosphaerae
8	8.5	-	Pseudomonas sp.
9	-	-	Psychrobacter sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	9	-	Pseudomonas sp.
6.6	-	remains stable at pH 6.6	Serratia marcescens
7	10	-	Psychrobacter sp.
8	-	stable at pH 8	Lysinibacillus sphaericus
8	-	stable at pH 8	Microbacterium phyllosphaerae