Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by EDTA and Tween 80 | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Pseudomonas aeruginosa | |
additional information | the enzyme is not affected by EDTA and Tween 80 | Pseudomonas aeruginosa | |
SDS | - |
Pseudomonas aeruginosa | |
Triton X-100 | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
4-nitrophenyl palmitate | pH 8.0, 30°C | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59400 | - |
x * 59400, SDS-PAGE | Pseudomonas aeruginosa |
300000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
isolated from the soil | - |
Pseudomonas aeruginosa MTCC 5113 | - |
isolated from the soil | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 21fold from the culture supernatant by acetone precipitation, anion exchange chromatography and gel filtration | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19280 | - |
purified enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa MTCC 5113 | 4-nitrophenol + palmitate | - |
? | |
trans-3-(4-methoxyphenyl) glycidic acid methyl ester + H2O | i.e. (+-)-methyl trans-3(4-methoxyphenyl) glycidate, the substrate is a key intermediate in the synthesis of cardiovascular drug, diltiazem | Pseudomonas aeruginosa | ? | - |
? | |
trans-3-(4-methoxyphenyl) glycidic acid methyl ester + H2O | i.e. (+-)-methyl trans-3(4-methoxyphenyl) glycidate, the substrate is a key intermediate in the synthesis of cardiovascular drug, diltiazem | Pseudomonas aeruginosa MTCC 5113 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 59400, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
enantiospecific lipase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
30°C, 48 h, purified enzyme, loss of 33% activity | Pseudomonas aeruginosa |
7 | 9 | 30°C, 48 h, purified enzyme, completely stable | Pseudomonas aeruginosa |
10 | - |
30°C, 3 h, purified enzyme, loss of 50% activity | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing | - |
5.5 |