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Literature summary for 3.1.1.3 extracted from

  • Eydoux, C.; De Caro, J.; Ferrato, F.; Boullanger, P.; Lafont, D.; Laugier, R.; Carriere, F.; De Caro, A.
    Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells (2007), J. Lipid Res., 48, 1539-1549.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Colipase
-
Homo sapiens
sodium taurodeoxycholate
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of PLRP2 in the protease A-deficient yeast Pichia pastoris as secreted enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates the phospholipase A1 activity of the enzyme, overview Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49946
-
x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens
50000
-
x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens
52343
-
x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant secreted enzyme from protease A-deficient Pichia pastoris strain culture supernatant by cation and anion exchange chromatography to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
550
-
purified recombinant enzyme in presence of colipase at pH 8.8 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows phospholipase A1 activity, EC 3.1.1.32, galactolipase activity, EC 3.1.1.26, and acylglycerol lipase activity, EC 3.1.1.23, overview Homo sapiens ?
-
?
olive oil + H2O
-
Homo sapiens ?
-
?
tributyrin + H2O
-
Homo sapiens dibutyrin + butyrate
-
?
trioctanoin + H2O
-
Homo sapiens dioctanoin + octanoate
-
?
triolein + H2O
-
Homo sapiens diolein + oleate
-
?

Subunits

Subunits Comment Organism
? x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
HPLRP2
-
Homo sapiens
pancreatic lipase-related protein 2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH profiles of the recombinant enzyme, overview Homo sapiens