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Literature summary for 3.1.1.3 extracted from

  • Fernandez-Lorente, G.; Palomo, J.M.; Cabrera, Z.; Fernandez-Lafuente, R.; Guisan, J.M.
    Improved catalytic properties of immobilized lipases by the presence of very low concentrations of detergents in the reaction medium (2007), Biotechnol. Bioeng., 97, 242-250.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CTAB PFL immobilized on glyoxyl and BrCN agarose is activates 8fold at 0.01% CTAB Pseudomonas fluorescens
additional information detergents mediate hyperactivation, effect of the detergents is to shift the conformational equilibrium of lipases toward the open form Pseudomonas fluorescens
additional information detergents mediate hyperactivation, effect of the detergents is to shift the conformational equilibrium of lipases toward the open form Moesziomyces antarcticus

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme on glyoxyl-agarose at pH 10.0 and 25°C and on BrCN agarose, overview Pseudomonas fluorescens
additional information immobilization of the enzyme on glyoxyl-agarose at pH 10.0 and 25°C and on BrCN agarose, overview Moesziomyces antarcticus

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride the presence of detergents, e.g. SDS, CTAB, Triton X-100, or Triton X-45, permitts the inhibition of lipase by irreversible covalent inhibitors, e.g. 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF, while the enzyme, in the absence of detergent, is not inhibited by these irreversible inhibitors Moesziomyces antarcticus
4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride the presence of detergents, e.g. SDS, CTAB, Triton X-100, or Triton X-45, permits the inhibition of lipase by irreversible covalent inhibitors, e.g. 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF, while the enzyme, in the absence of detergent, is not inhibited by these irreversible inhibitors Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Moesziomyces antarcticus
-
isozyme b
-
Pseudomonas fluorescens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Pseudomonas fluorescens
-
commercial preparation
-
Moesziomyces antarcticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities of the enzyme immobilized on glyoxyl-agarose and on BrCN agarose, overview Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+-)-2-hydroxy-4-phenylbutyric acid ethyl ester + H2O enantioselectivity of the immobilized enzyme in this reaction is increased 40fold by presence of 0.1% CTAB Pseudomonas fluorescens ?
-
?
4-nitrophenyl palmitate + H2O
-
Pseudomonas fluorescens 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Moesziomyces antarcticus 4-nitrophenol + palmitate
-
?

Synonyms

Synonyms Comment Organism
PFL
-
Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas fluorescens
25
-
assay at Moesziomyces antarcticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Pseudomonas fluorescens
7
-
assay at Moesziomyces antarcticus